Evaluation Of Periodontal Status In Gastritis Patients With Clinical And Microbiological Parameters
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 30
- 试验地点
- 1
- 主要终点
- Salivary microbial profile
研究概览
简要总结
The aim of this study to evaluate the microbial profile in gastritis and healthy patients in different periodontal conditions via next generation technologies.
详细描述
This study aims to determine the density of bacteria associated with periodontal disease and to evaluate oral dysbiosis in gastritis patients using metagenomic analysis.
研究设计
- 研究类型
- Observational
- 观察模型
- Cohort
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 20 Years 至 55 Years（Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •no systemic diseases other than gastritis non-smoker
排除标准
- •Participants who took regular medications that could affect of periodontal tissue or oral microbiome pregnancy or lactation Patients who received periodontal treatment within the last 6 months
研究组 & 干预措施
Gastritis
Patients diagnosed with gastritis via endoscopic method and without other systemic diseases
干预措施: saliva collection (Other)
Systemically healthy
Patients without any systemic disease
干预措施: saliva collection (Other)
结局指标
主要结局
Salivary microbial profile
时间窗: 1 month
To compare the oral flora profiles of the study groups, a microbiome-based next-generation sequencing approach targeting the 16S rRNA gene was employed. In this metagenomic study, following DNA extraction, phylogenetic classification was performed using the V3-V4 hypervariable regions of the 16S rRNA gene. Using the obtained data, total bacterial diversity and density were compared through alpha and beta diversity analyses. Bacterial taxa that differed between the groups were identified using LEfSe analysis.
次要结局
未报告次要终点
研究者
Veli Özgen Öztürk
Principal Investigator
Aydin Adnan Menderes University