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ABCB4 Gene Mutations in Intrahepatic Cholestasis of Pregnancy and Controls

Completed
Conditions
Intrahepatic Cholestasis of Pregnancy
Registration Number
NCT00700232
Lead Sponsor
University Hospital, Tours
Brief Summary

Mutations of the ATP binding cassette subfamily B member 4 (ABCB4) gene, a gene involved in a subtype of progressive familial intrahepatic cholestasis, have been reported in women suffering from intrahepatic cholestasis of pregnancy. The true incidence and the role of these ABCB4 gene mutations in patients suffering from intrahepatic cholestasis of pregnancy have not been clearly established.

The aim of the present study is to describe the nature and frequency of these mutations in a series of patients with intrahepatic cholestasis of pregnancy and to compare with a control group of pregnant women without intrahepatic cholestasis of pregnancy.

Detailed Description

Intrahepatic cholestasis of pregnancy was defined by pruritus and elevated serum alanine aminotransferase activity or bile acid concentration, with recovery after delivery. Patients with intercurrent liver disease were excluded.

The entire ABCB4 gene coding sequence and the promoter region were analyzed, during the routine medical management, by single strand conformation polymorphism and/or sequencing in 50 unrelated Caucasian patients with intrahepatic cholestasis of pregnancy.

The genomic variants detected in these patients with intrahepatic cholestasis of pregnancy will be sought in 100 control women from Caucasian origin recruited in the same hospital.

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
120
Inclusion Criteria
  • pregnant woman (age > 18 years) during the third trimester
  • caucasian and born in France
  • multiparous (with at least 2 full term pregnancies)
  • with informed written consent
Exclusion Criteria
  • generalized pruritus and/or liver disease during pregnancy
  • liver disease secondary to oral contraception
  • Chronic liver disease
  • disease during current pregnancy

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Service de Gynécologie Obstétrique CHRU Tours

🇫🇷

Tours, France

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