The Effects of Low Carbohydrate Diet on Liver Fat Content and Mitochondrial Fluxes
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 10
- 试验地点
- 2
- 主要终点
- Change in hepatic mitochondrial flux
研究概览
简要总结
The purpose of this study is to examine whether a low carbohydrate diet as compared to simple calorie restriction decreases hepatic lipid content and changes hepatic mitochondrial flux in humans.
详细描述
In this study, the investigators will examine whether a low carbohydrate diet as compared to simple calorie restriction will change hepatic lipid content and mitochondrial flux in humans. Before and after a low carbohydrate diet and a simple calorie restriction intervention, rates of hepatic mitochondrial flux will be assessed using positional isotopomer analysis of 3-13C-lactate during a 3 hour basal period. Rates of whole body ketogenesis will be determined using [13C4]β-hydroxybutyrate, and whole body glucose metabolism will be measured using [2H7]glucose. Body composition will be determined with bioelectrical impedance; hepatic lipid content will be measured with MRS. Whole-body oxidation of lipids, carbohydrates and protein will be determined using indirect calorimetry.
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 70 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Age 18-70
- •Subjects must be able to communicate meaningfully with the investigator and must be legally competent to provide written informed consent
排除标准
- •chronic liver disease other than NAFLD
- •chronic disease associated with hepatic steatosis such as diabetes mellitus
- •use of drugs known to affect hepatic steatosis
- •contraindications for MRI
- •pregnancy or nursing at the time of the study
结局指标
主要结局
Change in hepatic mitochondrial flux
时间窗: 1 week
Change in the rate of hepatic mitochondrial flux (Vcs) will be assessed using 3-13C-lactate infusion.
Change in hepatic triglyceride content
时间窗: 1 week
Change in hepatic triglyceride content will be measured using 1H magnetic resonance spectroscopy (MRS).
次要结局
- Change in beta-hydroxybutyrate production rate(1 week)
- Change in glucose production rate(1 week)
研究者
Panu Luukkonen
MD, PhD
Helsinki University Central Hospital