The Effects of Low Carbohydrate Diet on Liver Fat Content and Mitochondrial Fluxes
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Fatty Liver
- Sponsor
- Helsinki University Central Hospital
- Enrollment
- 10
- Locations
- 1
- Primary Endpoint
- Change in hepatic mitochondrial flux
- Status
- Active, not recruiting
- Last Updated
- last year
Overview
Brief Summary
The purpose of this study is to examine whether a low carbohydrate diet as compared to simple calorie restriction decreases hepatic lipid content and changes hepatic mitochondrial flux in humans.
Detailed Description
In this study, the investigators will examine whether a low carbohydrate diet as compared to simple calorie restriction will change hepatic lipid content and mitochondrial flux in humans. Before and after a low carbohydrate diet and a simple calorie restriction intervention, rates of hepatic mitochondrial flux will be assessed using positional isotopomer analysis of 3-13C-lactate during a 3 hour basal period. Rates of whole body ketogenesis will be determined using \[13C4\]β-hydroxybutyrate, and whole body glucose metabolism will be measured using \[2H7\]glucose. Body composition will be determined with bioelectrical impedance; hepatic lipid content will be measured with MRS. Whole-body oxidation of lipids, carbohydrates and protein will be determined using indirect calorimetry.
Investigators
Panu Luukkonen
MD, PhD
Helsinki University Central Hospital
Eligibility Criteria
Inclusion Criteria
- •Age 18-70
- •Subjects must be able to communicate meaningfully with the investigator and must be legally competent to provide written informed consent
Exclusion Criteria
- •chronic liver disease other than NAFLD
- •chronic disease associated with hepatic steatosis such as diabetes mellitus
- •use of drugs known to affect hepatic steatosis
- •contraindications for MRI
- •pregnancy or nursing at the time of the study
Outcomes
Primary Outcomes
Change in hepatic mitochondrial flux
Time Frame: 1 week
Change in the rate of hepatic mitochondrial flux (Vcs) will be assessed using 3-13C-lactate infusion.
Change in hepatic triglyceride content
Time Frame: 1 week
Change in hepatic triglyceride content will be measured using 1H magnetic resonance spectroscopy (MRS).
Secondary Outcomes
- Change in beta-hydroxybutyrate production rate(1 week)
- Change in glucose production rate(1 week)