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Establishment of human in vitro keratinocyte culture models for the study of dermatopharmacological and drug allergy issues

Recruiting
Conditions
Drug allergy
Z88
Personal history of allergy to drugs, medicaments and biological substances
Registration Number
DRKS00028025
Lead Sponsor
Bundesinstitut für Arzneimittel und MedizinprodukteAbteilung Forschung
Brief Summary

Not available

Detailed Description

Not available

Recruitment & Eligibility

Status
Recruiting
Sex
All
Target Recruitment
140
Inclusion Criteria

1) Tissue donations from patients: clinically documented association and confirmed diagnosis of drug allergy

2) Tissue donations from control subjects: no drug allergy

3) Donations of skin or hair: healthy skin or hair

4) Circumcidate donors: 0-17 years of age

5) Other tissue donors: 18-90 years of age

6) Written and verbal consent to participate, for minors by both legal guardians

Exclusion Criteria

1) Tissue donations from patients: No drug allergy (lack of temporal association and lack of evidence)

2) Tissue donations from control subjects: clinically documented connection and confirmed diagnosis of a drug allergy

3) Donations of skin or hair: pathologically altered skin or hair.

4) Circumcidate donors: >17 years of age

5) Other tissue donors: <18 years of age

6) Lack of capacity to give consent to participate in the study

7) Pregnancy

Study & Design

Study Type
observational
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Establishment of human in vitro keratinocyte culture models for the study of dermatopharmacological and drug allergy issues
Secondary Outcome Measures
NameTimeMethod
1) Development and characterization of human in vitro keratinocyte culture models 2) Isolation and characterization of drug/metabolite specific lymphocytes from peripheral blood of drug allergic patients 3) Etablierung eines funktionellen In-vitro-Hybridmodells, das eine arzneimittelinduzierte allergische Hautreaktion eines bereits sensibilisierten Patienten nachahmen soll
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