Exploratory study of the effect of oral hygiene and circadian rhythm on oral microbiota composition in healthy volunteers

Recruiting

Conditions: The aim is to analyse circadian oscillations of the oral microbiome, including F. nucleatum, and the effects of oral hygiene measures on the oral microbiome composition over time.

Registration Number: DRKS00023366

Lead Sponsor: German Cancer Research Center (DKFZ)

Brief Summary: Not available

Detailed Description: Not available

Recruitment & Eligibility

Status: Recruiting

Sex: All

Target Recruitment: 20

Inclusion Criteria

Persons who have understood the informed consent and signed the written consent form.

Exclusion Criteria

- Rejection by the study participant
- Pregnancy or nursing mothers
- Lack of ability to consent
- Persons who are currently taking or have taken antibiotics in the last 3 weeks
- Infections of the oral mucosa, respiratory tract and GI tract in the last three weeks
- Test persons with tumor diseases, or chronic inflammatory intestinal diseases
- Subjects who are not able to fully consume diets orally
- Underweight or obesity
- Persons who received dentures or dental implants during the last 8 weeks
- Dental treatments in the last 3 weeks

Study & Design

Study Type: observational

Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Exploratory, longitudinal assessment of the diurnal variation of the relative abundance of Fusobacterium nucleatum as a member of the oral microbiota in a cohort of healthy volunteers with and without oral and dental hygiene.

Secondary Outcome Measures

Name	Time	Method
- Characterization of oral microbiota using metagenome sequencing to determine the diurnal variation of species, microbial genes, and metabolic pathways.<br>- Characterization of the stool microbiota at the end of each oral microbiota collection period.