Arginine Synthesis in Healthy Adult Humans: Does Proline or Glutamate Provide the 5-Carbon Chain?
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 6
- 试验地点
- 1
- 主要终点
- Blood Isotope Levels
研究概览
简要总结
Arginine synthesis in mammals is a complex process involving at least 8 enzymes and intracellular and interorgan transfer of substrates.
The investigators have shown in our neonatal studies that there is no conversion of glutamate to arginine but proline label appears in ornithine, citrulline and arginine. It is clear therefore that arginine is synthesized in human neonates from proline.
In adults the source of the carbon backbone is less clear.
详细描述
Through a series of test in healthy adults using tandem mass spectrometry with carbon labeled GLN and nitrogen labeled PRO to determine the dietary source for the carbon chain of ORN, CIT and ARG. Additionally, using Nitrogen labeled GLN, we will identify the source of the nitrogen atoms in these amino acids.
Using guanidino - 15N2 arginine, 1-13C Glutamine, 2-15N Glutamine and 15N Proline we will be able to:
- determine the flux of arginine and proline in healthy adult volunteers
- determine and compare the flux of carbon and nitrogen labeled glutamine
- demonstrate whether glutamine or proline provides the carbon backbone for ornithine, citrulline and arginine in adults.
- the source of the N atoms in ornithine, citrulline and arginine.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Single Group
- 主要目的
- Diagnostic
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •5 men of average weight 80 kg will be studied
- •healthy, with no know disease of liver, and/or kidney
- •weight stable
排除标准
- •weith loss or gain over the last three months
- •on medications known to affect amino acid metabolism
- •known liver, kidney disease condition
研究组 & 干预措施
Test Study 2: 15N2 arginine &15N proline
administration of 15N2 arginine &15N proline to measure arginine and proline flux
干预措施: 15N2 arginine and 15N proline (Other)
Test Study 1: 2-15N glutamine
To determine the contributions make by glutamine to the provision of nitrogen for ornithine, citrulline and arginine syntesis in adults.
干预措施: 2-15N glutamine (Other)
Study Test 3: 1-13C glutamine
to determine to contribution of glutamine to the carbon skeleton of ornithine, citrulline and arginine in adults.
干预措施: 1-13C glutamine (Other)
结局指标
主要结局
Blood Isotope Levels
时间窗: 8 hours
次要结局
未报告次要终点
研究者
Dr. Paul Pencharz
Emeritus Scientist
The Hospital for Sick Children