Biomarkers in Endometriosis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 100
- 主要终点
- Derivation of the panel of proteins indicative of endometriosis using comparative proteomics
研究概览
简要总结
Endometriosis is a common gynecological benign disease which significantly compromise quality of life in patients and is a major cofactor of infertility. With the proteomic approach the investigators will search for proteins with significantly different levels in peritoneal fluid, serum and uterine fluid samples of women with endometriosis versus control group. Results will be validate using ELISA aproach.
详细描述
Informed consent will be obtained from all individual participants included in the study. Blood sample will be obtained the day before laparoscopic surgery during regular clinical preoperative procedures. Peritoneal fluid and uterine fluid will be obtained during regular laparoscopic procedure indicated for infertility, the exact protocol of sample obtaining will be revealed after publication of results. The aim of the study was to identify potential diagnostic and predictive biomarkers or drug targets.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 18 Years 至 35 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •primary infertility
- •normal semen analysis of the partner
- •normal BMI.
排除标准
- •hormonal therapy in the last year,
- •irregular menstrual cycle,
- •previous pelvic surgery,
- •history of previous pelvic inflammatory disease,
- •smokers and patients with autoimmune diseases,
- •malignant or suspected malignant diseases,
- •previous pelvic inflammatory disease, leiomyoma uteri or polycystic ovaries
结局指标
主要结局
Derivation of the panel of proteins indicative of endometriosis using comparative proteomics
时间窗: 3 years to collect all speciments
The aim of the study is to identify proteins with different levels in cases versus controls these proteins could be new candidates for biomarkers of endometriosis. Differentially expressed proteins are candidates for diagnostic and predictive biomarkers. To identify differentially expresed proteins in discovery phase of the study proteomic aproach will be used.
次要结局
- Concentrations of proteins in body fluids to validate the identified panel of proteins indicative of endometriosis(3 years to collect all speciments)
研究者
Vid Jansa
Principal Investigator
University Medical Centre Ljubljana