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Dose and Response of Dietary Nitrate in Healthy and Active Men

Not Applicable
Completed
Conditions
Healthy
Interventions
Dietary Supplement: sodium nitrate
Dietary Supplement: Beetroot juice
Registration Number
NCT01961427
Lead Sponsor
Swiss Paraplegic Research, Nottwil
Brief Summary

It was shown by some researchers that dietary nitrate such as beet root juice can lower oxygen consumption in men and act as a performance enhancing supplement.

It was not shown, what the optimal dosage is and if there is any difference in oxygen consumption comparing beet root juice and inorganic nitrate.

Therefore, the aim of the study is to conduct a study ingesting different sodium nitrate and beet root juice dosages. We would measure nitrate and nitrite concentrations in the blood at different time points and the subjects have to perform an exercise test measuring oxygen consumption 3 hours after the ingestion of nitrate.

Detailed Description

1. The subjects have to ingest 7 different dosages of either beetroot juice or sodium nitrate on seven different occasions. Every hour a blood withdrawal is taken to measure nitrate and nitrite concentration in the blood.

2. The subjects have to ingest 7 different dosages of either beetroot juice or sodium nitrate on seven different occasions. Three hours after the intake of the supplement the subjects have to perform an exercise test on a bicycle. They have to cycle 5 min at 50% of maximal oxygen consumption and 8min at 80% of maximal oxygen consumption separated by a 5min break. Oxygen consumption would be measured during the whole exercise test. Blood withdrawal is taken before the ingestion, after 3 hours and after the test.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
21
Inclusion Criteria
  • age 18 to 45
  • healthy active men
  • non-smoking
Exclusion Criteria
  • medication intake during study phase

Study & Design

Study Type
INTERVENTIONAL
Study Design
FACTORIAL
Arm && Interventions
GroupInterventionDescription
sodium Nitrate (6mmol)sodium nitrateingestion of inorganic nitrate (6mmol solution)
sodium nitrate 3mmolsodium nitrateingestion of inorganic nitrate (3mmol solution)
Beetroot juice (12mmol)Beetroot juiceingestion of 170ml of concentrated beetroot juice (12mmol)
Beetroot juice (3mmol)Beetroot juiceIngestion of 42.5ml of concentrated beetroot juice (3mmol)
sodium nitrate (0mmol)sodium nitrateIngestion of water as a placebo
sodium Nitrate 12mmolsodium nitrateIngestion of a solution of inorganic nitrate, dosage: 12mmol
beetroot juice (6mmol)Beetroot juiceingestion of 85ml concentrated beetroot juice (6mmol)
Primary Outcome Measures
NameTimeMethod
oxygen consumption30min

Oxygen consumption during exercise protocol

Secondary Outcome Measures
NameTimeMethod
Blood pressure6 hours

every hour, blood pressure is measured.

Nitrate and nitrite concentration6 hours

every hour for the six hours after the ingestion of dietary/inorganic nitrate, nitrate and nitrite concentration in the blood is analyzed.

oxygen saturation6 hours

every hour oxygen saturation is measured.

Rating of perceived exertion (RPE)during the 7 weeks of study phase

RPE is measured after the low-intensity exercise performance and after the submaximal exercise performance.

Heart rateduring the 7 weeks of study phase

Heart rate is measured before the ingestion, before the exercise test and during the exercise test. resting heart rate is additionally measured every hours after the intake of dietary/inorganic nitrate.

Trial Locations

Locations (1)

Swiss Paraplegic Centre

🇨🇭

Nottwil, Lucerne, Switzerland

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