Epigenetic Reprogramming of Monocytes in Patients With Coronary Atherosclerosis
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 40
- 试验地点
- 1
- 主要终点
- Epigenetic modifications in circulating monocytes
研究概览
简要总结
In this study, the investigators will determine whether patients with documented atherosclerosis are characterized by specific epigenetic changes in circulating cells of the innate immune system, compared to patients without atherosclerosis.
详细描述
Rationale: The innate immune system plays a pivotal role in the development and progression of atherosclerosis. Recently, it was reported that monocytes can develop a long-lasting immunological memory after stimulation with various microorganisms, which has been termed 'trained innate immunity'. This memory is induced by epigenetic reprogramming. We hypothesize that trained monocytes augment atherogenesis.
Objective
The main objective is to study whether patients with coronary atherosclerosis show specific epigenetic changes in the promoter regions of pro-inflammatory cytokines and chemokines and whether this correlates with the inflammatory phenotype of these cells.
Study design: Observational study
Study population: Adult patients who have had computed tomography coronary angiography because of chest pain: 20 patients without atherosclerosis and 20 patients with severe atherosclerosis will be included.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- 18 Years 至 —（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •Patients (male/female),
- •aged >18 years,
- •admitted to the acute coronary unit of the CWZ hospital for evaluation of chest pain.
- •No coronary atherosclerosis
- •Or Severe atherosclerosis
排除标准
- •Signs or symptoms of a current infection (fever, chills)
- •Auto-immune diseases (such as rheumatoid arthritis)
结局指标
主要结局
Epigenetic modifications in circulating monocytes
时间窗: baseline
次要结局
- Inflammatory phenotype(baseline)