Investigation of the Effects of Sedentary Behaviour and Moderate Exercise on Glucose Tolerance and Insulin Sensitivity
- Conditions
- Glucose MetabolismInsulin SensitivitySedentary Lifestyle
- Interventions
- Other: Activity/Moderate Exercise
- Registration Number
- NCT03827239
- Lead Sponsor
- University of Prince Edward Island
- Brief Summary
Individuals with pre-diabetes or diabetes would benefit from low impact methods that would improve their insulin sensitivity and aid in maintaining glucose homeostasis. Physical activity helps the body decrease its insulin resistance and burn excess sugar. Many diabetics also suffer from obesity and specific forms or durations of physical exercise may not be viable options for these individuals. Determining whether short bursts of moderate exercise improve blood glucose levels in healthy humans may identify a further method for diabetics to improve their glucose homeostasis.
The aim of this study is to determine the most effective moderate exercise that can be completed in 3 minutes and its effectiveness on improving glucose handling, in response to a single day of standardized high-fat and high-carbohydrate feeding in comparison to periods of prolonged sitting.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- TERMINATED
- Sex
- All
- Target Recruitment
- 6
- healthy
- BMI between 18-30 kg/m²
- non-smoker
- pregnant
- diabetes
- kidney disease
- liver disease
- cardiovascular disease
- cancer
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- CROSSOVER
- Arm && Interventions
Group Intervention Description Intervention Activity/Moderate Exercise Will disrupt their sedentary time with 3 minute exercise sessions every 30 minutes
- Primary Outcome Measures
Name Time Method Change in metabolomic response to a test meal Pre-prandial and 3-hours post test meal through study completion Targeted metabolomics analysis of approximately 139 metabolites in blood serum by LC-MS/MS to generate a metabolomic profile in response to a high-fat and high-carbohydrate test meal. Metabolites to be determined are categorized as biogenic amines, amino acids, histidines, carboxylic acids, acylcarnitines, glycerophospholipids and organic acids.
Change in concentration of C-peptide Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days The change in blood C-peptide levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).
Change in Insulin levels Pre-prandial baseline (12 hours fasted) and 3 hours post-prandial on each of two testing days The change in blood insulin levels from baseline (12 hours fasted) to 3-hours post-prandial will be determined from blood serum using a human metabolic hormone multiplex panel (Luminex xMAP technology).
Changes in blood glucose levels Baseline and every 30-60 minutes over 3 hours (6 timepoints per test day) Blood glucose levels will be determined at pre-prandial baseline and over the following 3-hour testing period using hand-held blood glucose meters.
- Secondary Outcome Measures
Name Time Method Immediate effects of short-term sedentary behaviour on cognitive function 3 hours post-activity (sedentary behaviour or exercise) on test days through study completion Assessing cognitive abilities using the Flankers test.
Gene expression levels in isolated PBMC populations Pre-prandial and 3 hours post-prandial on on test days through study completion Determining changes in gene expression in pathways associated with RAGE signalling.
Trial Locations
- Locations (1)
University of Prince Edward Island
🇨🇦Charlottetown, Prince Edward Island, Canada