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A Trial Investigating the Dose Linearity and Safety of BC Combo THDB0207 in Subjects With Type 2 Diabetes

Phase 1
Completed
Conditions
Type 2 Diabetes
Interventions
Drug: Euglycemic clamp with BC Combo THDB0207
Drug: Euglycemic clamp with Humalog® Mix25
Registration Number
NCT05373212
Lead Sponsor
Adocia
Brief Summary

This is a randomised, double-blind, four-period crossover euglycaemic clamp trial in subjects with type 2 diabetes.

Each subject will be randomly allocated to one of four treatment sequences. Each sequence will comprise 3 different single doses of BC Combo THDB0207 (Low dose, Medium dose, and High dose) and one single dose of Humalog® Mix25.

Subjects will come to the clinical trial centre in a fasted state in the morning of each dosing day and stay at the clinical trial centre until the 30-hour clamp procedures have been terminated.

Detailed Description

Subjects will attend the study site in the morning in a fasted state and will be connected to an automated glucose clamp device. Prior to dose administration plasma glucose will be stabilised at a target level of 100 mg/dL by means of an intravenous infusion of glucose or insulin. IMP administration will be done by an unblinded person by means of subcutaneous injections in the abdominal wall.

Following each dosing a euglycaemic glucose clamp procedure will be carried out for up to 30 hours.

The pharmacodynamic assessment will be based on the time course of glucose infusion rate (GIR) and plasma glucose.

Plasma insulin concentrations will be measured using a specific validated bioanalytical method differentiating concentrations of insulin glargine, of its main metabolites insulin-glargine-M1 and insulin-glargine-M2, and of insulin lispro. Pharmacokinetic assessments will be based on total insulin concentration (insulin glargine + insulin glargine-M1 + insulin glargine-M2 + insulin lispro), on insulin glargine concentration (insulin glargine + insulin glargine-M1 + insulin glargine-M2), or on insulin lispro concentration.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
40
Inclusion Criteria
  • Type 2 diabetes mellitus (as diagnosed clinically) for ≥ 12 months
  • HbA1c ≤9.0%
  • Total insulin dose of < 1.2 U/kg/day
  • Body mass index between 20.0 and 35.0 kg/m2 (both inclusive)
  • Treated with a stable insulin regimen for ≥ 3 months prior to screening
Exclusion Criteria
  • Known or suspected hypersensitivity to the IMPs or any of the excipients or to any component of the IMP formulation
  • Receipt of any medicinal product in clinical development within 30 days or at least 5 half-lives of the related substances and their metabolites (whichever is longer) before randomisation in this trial
  • Clinically significant abnormal screening laboratory tests, as judged by the Investigator considering the underlying disease
  • Clinically relevant comorbidity, capable of constituting a risk for the subject when participating in the trial or of interfering with the interpretation of data
  • Systolic blood pressure < 90 mmHg or >160 mmHg and/or diastolic blood pressure < 50 mmHg or > 95 mmHg (one repeat test will be acceptable in case of suspected white-coat hypertension)
  • Heart rate at rest outside the range of 50-90 beats per minute
  • Use of GLP-1 receptor agonists or oral antidiabetic drugs (OADs) other than stable intake of metformin within 4 weeks prior to screening
  • Women of childbearing potential who are not using a highly effective contraceptive method

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
BC Combo THDB0207 Medium doseEuglycemic clamp with BC Combo THDB0207Single administration of BC Combo THDB0207 (Medium dose)
BC Combo THDB0207 Low doseEuglycemic clamp with BC Combo THDB0207Single administration of BC Combo THDB0207 (Low dose)
BC Combo THDB0207 High doseEuglycemic clamp with BC Combo THDB0207Single administration of BC Combo THDB0207 (High dose)
Humalog® Mix25Euglycemic clamp with Humalog® Mix25Single administration of Humalog® Mix25
Primary Outcome Measures
NameTimeMethod
AUCTOTAL0-lastFrom t=0 to t=30 hours after IMP administration

Area under the total insulin concentration-time curve from t=0 to the last measured insulin concentration above LLOQ

CmaxTOTALFrom t=0 to t=30 hours after IMP administration

Maximum total insulin concentration

Secondary Outcome Measures
NameTimeMethod
AUCTOTAL 12-24hFrom t=12 to t=24 hours

Area under the total insulin concentration-time curve from t=12 to t=24 hours

AUCLIS 12-24hFrom t=12 to t=24 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=12 to t=24 hours

AUCGIR 0-lastFrom t=0 to t=30 hours after IMP administration

Area under the glucose infusion rate curve from 0 hours until the end of clamp

GIRmaxFrom t=0 to t=30 hours after IMP administration

Maximum glucose infusion rate

AUCTOTAL 6-24hFrom t=6 to t=24 hours

Area under the total insulin concentration-time curve from t=6 to t=24 hours

AUCTOTAL 0-2hFrom t=0 to t=2 hours

Area under the total insulin concentration-time curve from t=0 to t=2 hours

AUCTOTAL 0-6hFrom t=0 to t=6 hours

Area under the total insulin concentration-time curve from t=0 to t=6 hours

AUCTOTAL 12-30hFrom t=12 to t=30 hours

Area under the total insulin concentration-time curve from t=12 to t=30 hours

AUCTOTAL 0-30hFrom t=0 to t=30 hours

Area under the total insulin concentration-time curve from t=0 to t=30 hours

CTOTALmaxFrom t=0 to t=30 hours after IMP administration

Maximum insulin concentration

tmaxTOTALFrom t=0 to t=30 hours after IMP administration

Time to maximum total insulin concentration

AUCGLA 0-lastFrom t=0 to t=30 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=0 to the last measured insulin concentration above LLOQ

AUCGLA 2-6hFrom t=2 to t=6 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=2 to t=6 hours

AUCGLA 12-24hFrom t=12 to t=24 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=12 to t=24 hours

AUCGLA 0-30hFrom t=0 to t=30 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=0 to t=30 hours

Tonset of actionFrom t=0 to t=30 hours after IMP administration

Time until Plasma Glucose (PG) has decreased by at least 5 mg/dL from the baseline PG value.

AUCTOTALlastFrom t=0 to t=30 hours after IMP administration

Area under the insulin concentration-time curve from t=0 to the last measured insulin concentration above LLOQ

AUCTOTAL 0-1hFrom t=0 to t=1 hour

Area under the total insulin concentration-time curve from t=0 to t=1 hour

AUCGLA 12-30hFrom t=12 to t=30 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=12 to t=30 hours

AUCGIR 0-6hFrom t=0 to t=6 hours

Area under the glucose infusion rate curve from t=0 hours to t=6 hours

AUCGLA 0-6hFrom t=0 to t=6 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=0 to t=6 hours

AUCLIS0-lastFrom t=0 to t=30 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=0 to the last measured insulin concentration above LLOQ

AUCLIS 0-1hFrom t=0 to t=1 hour after IMP administration

Area under the insulin lispro concentration-time curve from t=0 to t=1 hour

AUCLIS 0-2hFrom t=0 to t=2 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=0 to t=2 hours

AUCLIS 0-6hFrom t=0 to t=6 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=0 to t=6 hours

AUCLIS 2-6hFrom t=2 to t=6 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=2 to t=6 hours

tGIRmaxFrom t=0 to t=30 hours after IMP administration

Time to maximum glucose infusion rate

AUCTOTAL 2-6hFrom t=2 to t=6 hours

Area under the total insulin concentration-time curve from t=2 to t=6 hours

AUCTOTAL 6-12hFrom t=6 to t=12 hours

Area under the total insulin concentration-time curve from t=6 to t=12 hours

AUCGLA 0-1hFrom t=0 to t=1 hour after IMP administration

Area under the insulin glargine concentration-time curve from t=0 to t=1 hour

AUCGLA 0-2hFrom t=0 to t=2 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=0 to t=2 hours

AUCGLA 6-12hFrom t=6 to t=12 hours after IMP administration

Area under the insulin glargine concentration-time curve from t=6 to t=12 hours

CmaxGLAFrom t=0 to t=30 hours after IMP administration

Maximum concentration of insulin glargine

tmaxGLAFrom t=0 to t=30 hours after IMP administration

Time to maximum insulin glargine concentration

CmaxLISFrom t=0 to t=30 hours after IMP administration

Maximum concentration of insulin lispro

AUCLIS 6-12hFrom t=6 to t=12 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=6 to t=12 hours

AUCLIS 12-30hFrom t=12 to t=30 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=12 to t=30 hours

AUCLIS 0-30hFrom t=0 to t=30 hours after IMP administration

Area under the insulin lispro concentration-time curve from t=0 to t=30 hours

Adverse EventsFrom the first IMP administration to the follow-up visit (i.e. up to 14 weeks)

Incidence of Adverse Events

tmaxLISFrom t=0 to t=30 hours after IMP administration

Time to maximum insulin lispro concentration

Local tolerabilityFrom the first IMP administration to the follow-up visit (i.e. up to 14 weeks)

Incidence of Injection Site Reactions

Trial Locations

Locations (1)

Profil Institut für Stoffwechselforschung GmbH

🇩🇪

Neuss, Germany

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