Detection of Salivary Insulin Following Low Versus High Carbohydrate Meals in Humans
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 16
- 试验地点
- 2
- 主要终点
- Peak plasma insulin level
研究概览
简要总结
In addition to its role as the major regulator of glucose uptake into peripheral tissues, the hormone insulin is also a primary regulator of fat storage and fat burning of the body. Mechanistic animal studies have shown that high insulin may be the initial trigger for weight gain and be the proximal cause of obesity. There is currently no quick or non-invasive way of measuring insulin as research and clinical techniques require collection of a blood sample followed by a complicated and costly biochemical assay. The investigators will test the hypothesis that insulin levels can be accurately measured in saliva in humans following meals that elicit high and low blood insulin responses. Findings will help determine if insulin can be use as a valid fluid to track insulin changes in humans.
详细描述
Lean and obese healthy participants will consume both a high carbohydrate meal and a low carbohydrate meal designed to elicit different insulin responses on two separate occasions. Blood samples and saliva samples will be collected for 2 hours following each meal and insulin will be measured in both plasma and saliva. The relationship between plasma and saliva insulin following the two meals will be assessed.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Body mass index between 18.5-24.9 or over 30.0 kg/m2
排除标准
- •Diabetes or other medical conditions affecting glucose or insulin levels
- •Competitive athlete
- •Vegan or vegetarian
结局指标
主要结局
Peak plasma insulin level
时间窗: 0-120 minutes
Peak plasma insulin concentration in high carbohydrate versus low carbohydrate arm.
次要结局
- Timing of peak saliva and peak plasma insulin(0-120 minutes)
- Plasma insulin area under the curve(0-120 minutes)
- Saliva insulin area under the curve(0-120 minutes)
- Relationship between peak saliva and peak plasma insulin(0-120 minutes)
- Relationship between saliva insulin area under the curve and plasma insulin area under the curve(0-120 minutes)
- Peak saliva insulin level(0-120 minutes)