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A Study to Evaluate A Range of Dose Levels of Ad26.ZEBOV and MVA-BN-Filo in Healthy Adult Participants

Phase 3
Completed
Conditions
Healthy
Interventions
Biological: Ad26.ZEBOV 2*10^10 (vp)
Biological: Ad26.ZEBOV 0.8*10^10 (vp)
Biological: Ad26.ZEBOV 5*10^10 viral particles (vp)
Biological: Placebo
Biological: MVA-BN-Filo 1*10^8 Infectious Unit [Inf. U.]
Biological: MVA-BN-Filo 5*10^7 Inf. U.
Registration Number
NCT02543567
Lead Sponsor
Janssen Vaccines & Prevention B.V.
Brief Summary

The purpose of this study is to demonstrate the non-inferiority of a heterologous prime-boost regimen using Ad26.ZEBOV as prime and MVA-BN-Filo as boost administered at different doses at a 56-day interval versus the same regimen with the recently released batches of Ad26.ZEBOV and MVA-BN-Filo in terms of humoral immune response against the Ebola virus (EBOV) GP (glycoprotein) as measured by enzyme-linked immunosorbent assay (ELISA) at 21 days post boost.

Detailed Description

This is a randomized, double-blind, placebo-controlled, parallel-group, multicenter study to evaluate safety and immunogenicity of Ad26.ZEBOV and MVA-BN-Filo at different dose levels, administered to healthy adults participants. The study consists of a Screening period of up to 6 weeks, vaccinations on Day 1 and Day 57, and a post-vaccination phase until the 6 months post-boost visit (Day 237). The participants will be randomized at baseline (on Day 1) in a 2:2:2:1 ratio to Groups 1, 2, 3 and 4. Safety will be monitored throughout the study.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
525
Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Group 1MVA-BN-Filo 1*10^8 Infectious Unit [Inf. U.]Ad26.ZEBOV 5\*10\^10 viral particles (vp) single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 1\*10\^8 Infectious Unit \[Inf. U.\] single dose IM injection on Day 57
Group 2Ad26.ZEBOV 2*10^10 (vp)Ad26.ZEBOV 2\*10\^10 vp single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 5\*10\^7 Inf. U single dose IM injection on Day 57
Group 3MVA-BN-Filo 5*10^7 Inf. U.Ad26.ZEBOV 0.8\*10\^10 vp single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 5\*10\^7 Inf. U. single dose IM injection on Day 57
Group 3Ad26.ZEBOV 0.8*10^10 (vp)Ad26.ZEBOV 0.8\*10\^10 vp single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 5\*10\^7 Inf. U. single dose IM injection on Day 57
Group 2MVA-BN-Filo 5*10^7 Inf. U.Ad26.ZEBOV 2\*10\^10 vp single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 5\*10\^7 Inf. U single dose IM injection on Day 57
Group 1Ad26.ZEBOV 5*10^10 viral particles (vp)Ad26.ZEBOV 5\*10\^10 viral particles (vp) single dose intramuscular (IM) injection on Day 1; MVA-BN-Filo 1\*10\^8 Infectious Unit \[Inf. U.\] single dose IM injection on Day 57
Group 4PlaceboParticipants will receive intramuscular (IM) injection of Placebo (0.9% saline) once on Day 1 and Day 57
Primary Outcome Measures
NameTimeMethod
Immune Responses to the Study Vaccine Regimens against Ebola virus (EBOV) Glycoprotein (GP) using EBOV GP protein Enzyme-linked Immunosorbent Assay (ELISA)At 21 days post boost vaccination

The humoral immune response will be assessed by enzyme-linked immunosorbent assay (ELISA) binding antibody

Secondary Outcome Measures
NameTimeMethod
Number of Participants with Solicited Local and Systemic Adverse Events (AEs)Up to 7 days after each vaccination
Number of Participants with Serious Adverse Events (SAEs)Continuous throughout the duration of study (Up to Day 237)
Number of Participants with Adverse Events (AEs)Up to 42 days post boost vaccination
Immune Responses to the Study Vaccine Regimens against Ebola virus (EBOV) Glycoprotein (GP) using EBOV GP protein Enzyme-linked Immunosorbent Assay (ELISA)At Days 1, 29, 57 post prime dose and at days 42, and 180 post boost vaccination

The humoral immune response will be assessed by enzyme-linked immunosorbent assay (ELISA) binding antibody

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