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Time Restricted Eating and Innate Immunity

Not Applicable
Completed
Conditions
Atherosclerotic Cardiovascular Disease
Time Restricted Feeding
Myocardial Infarction
Interventions
Behavioral: Time restricted eating (TRE)
Behavioral: Regular diet
Registration Number
NCT05639244
Lead Sponsor
Radboud University Medical Center
Brief Summary

The goal of this cross over study is to investigate the effect of short term time restricted eating (TRE) on the innate immune system in patients with a history of myocardial infarction.

Detailed Description

In the recent years, research has shown the prominent role of low grade systemic inflammation in cardiovascular disease (CVD) and the crucial role myeloid cells, mainly monocytes and macrophages, play in atherogenesis. Time restricted eating (TRE), i.e. eating the normal amount of calories within a limited time period per day, has a beneficial effect on multiple factors involved in the development of CVD, such as blood pressure, heart rate, lipid and blood glucose levels, and insulin sensitivity. TRE also reduces markers of systemic inflammation and reduces the number of circulating monocytes.

It is now hypothesized that TRE reduces the pro-inflammatory monocyte phenotype of patients with a history of myocardial infarction. Therefore, the investigators will perform a exploratory prospective randomised open label blinded endpoint cross-over study to investigate the effect of short term TRE on the innate immune system in patients with a history of myocardial infarction.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
23
Inclusion Criteria
  • Adult (age >18 years)
  • Diagnosed with a myocardial infarction (between 1 and 5 years ago)
  • Body mass index between 20 and 35 kg/m2
  • Able to understand, be motivated and follow the study related procedures
  • Able to understand and give written informed consent
Exclusion Criteria
  • Myocardial infarction (defined as an increase in cardiac enzymes in combination with symptoms of ischemia or newly developed ischemic ECG changes), coronary artery bypass graft surgery or other major (cardiovascular) surgery, stroke or transient ischemic attack (TIA) in the past 1 year prior to screening.
  • Use of immunomodulatory drugs
  • Use of drugs that need to be taken with food.
  • Diabetes Mellitus type I and type II
  • Medical history of any disease associated with immune deficiency (either congenital or acquired, including chemotherapy, active malignancy, organ transplant) or auto immune disease
  • Clinically significant infections within 1 months prior to start of or during intervention period or control period (defined as fever >38.5).
  • Vaccination <1 month before start of or during intervention or control period.
  • Eating disorders

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
First regular diet, then TRETime restricted eating (TRE)This group will start with the 2 week period of consuming their regular diet and will switch to 2 weeks TRE after a wash-out period.
First TRE, then regular dietTime restricted eating (TRE)This group will start with 2 weeks TRE and will switch to the 2 week period of consuming their regular diet after a wash-out period.
First TRE, then regular dietRegular dietThis group will start with 2 weeks TRE and will switch to the 2 week period of consuming their regular diet after a wash-out period.
First regular diet, then TRERegular dietThis group will start with the 2 week period of consuming their regular diet and will switch to 2 weeks TRE after a wash-out period.
Primary Outcome Measures
NameTimeMethod
The change in the inflammatory phenotype of circulating immune cells, assessed by measuring the cytokine production capacity (e.g. IL-1b and TNF) of isolated PBMCs after ex vivo stimulation with various TLR ligands, determined by ELISA.Change from baseline cytokine production capacity at 2 weeks after TRE or regular diet.

The investigators will measure the change in the inflammatory phenotype of circulating immune cells after TRE.

Therefore, the investigators will assess the inflammatory phenotype at baseline and after a two week TRE period and a control period with a regular diet (cross-over design).

This will be assessed by measuring the cytokine production capacity of isolated peripheral blood mononuclear cells (PBMCs) after ex vivo stimulation with various TLR ligands. Relevant cytokines (e.g. IL-1b and TNF, given in pg/ml) are measured in the supernatants of the PMBC stimulation experiments by ELISA.

Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

Radboud university medical center

🇳🇱

Nijmegen, Gelderland, Netherlands

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