Evaluation and Correlation of Serum Fetuin-A Levels in Periodontal Health and Disease: A Clinico-biochemical Study

Not Applicable

Completed

• Conditions: Gingivitis and Periodontal Diseases
• Interventions: Procedure: Non surgical periodontal therapy

• Registration Number: NCT04198610
• Lead Sponsor: Krishnadevaraya College of Dental Sciences & Hospital

Brief Summary

The present clinico-biochemical study was carried out to estimate the levels of Fetuin A and MMP7 in serum of systemically healthy subjects in periodontal health and disease and to evaluate the impact of periodontal interventional therapy (scaling and root planing) on the same.

Detailed Description

Sixty sex-matched subjects (30 males and 30 females) belonging to a common age group (30-39 yrs) were enrolled for the study to eliminate bias from confounding factors. They were divided into three groups consisting of 20 subjects in each group based on the clinical and radiological parameters; Periodontally healthy (Group I), Chronic gingivitis (Group II) and chronic periodontitis ( Group IIIa) from whom serum samples were collected. The Group IIIb further comprised of the subjects from Group IIIa, who had received non-surgical periodontal therapy (scaling and root planing). Serum samples were collected at baseline and at 12 weeks post therapy. Fetuin A and MMP-7 concentration was determined from serum samples of patients using ELISA. The results of our study depicted that Fetuin A concentration negatively correlates, whereas, MMP7 positively correlates with the extent of periodontal inflammation. Further, Periodontal therapy demonstrated a significant increase in serum Fetuin A and decrease in MMP 7 levels establishing the influence of periodontal therapy in stabilizing their levels.

Study Timeline

• Study Start: 2017-03-10
• Primary Completion: 2017-08-28
• Study Completion: 2017-08-28
• Status Verified: 2019-02
• Study First Submitted: 2019-02-26
• Study First Submitted QC: 2019-12-11
• Last Update Submitted: 2019-12-11
• Study First Posted: 2019-12-13
• Last Update Posted: 2019-12-13

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 60

Inclusion Criteria

1. Age group of 30-39 years.
2. Systemically healthy subjects.
3. Patient who are co-operative and able to attend follow up.
4. Patient who have not received any periodontal treatment within the past six months of baseline examination.
5. Dentition with at least 20 functional teeth.

Exclusion Criteria

1. Medically compromised patients (history of diabetes mellitus, liver diseases, cardiovascular disease, kidney diseases, rheumatoid arthritis, bone diseases pulmonary disease, viral and fungal infections).
2. Patients with calcification disorders (chronic kidney disease with vascular calcification, hyperparathyroidism, excessive intake of vitamin D, valvular calcific aortic stenosis
3. Aggressive periodontitis.
4. Bleeding disorders
5. Gross oral pathology and suppuration
6. Patients who had received antibiotic therapy and anti inflammatory within the last six months.
7. Anomalies of the immune system.
8. Pregnancy and lactation.

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
chronic periodontitis	Non surgical periodontal therapy	Chronic periodontitis was defined as having probing depth more than or equal to 5mm, RAL more than or equal to 8mm, with more than or equal to 10% sites with BOP positive and evidence of bone loss determined radiographically. Non surgical periodontal therapy (SRP) was concluded in 3 months. Within the duration of the study, all subjects received supportive therapy

Primary Outcome Measures

Name	Time	Method
estimate the change in levels of Fetuin A and MMP7 in serum at baseline and 3 months post SRP	Serum collection at baseline and 3 months	Serum was collected at baseline and 3 months and subjected to ELISA

Secondary Outcome Measures

Name	Time	Method
Modified Gingival index (GI)	At baseline and 3 months	The index was recorded at only baseline for healthy and gingivitis groups and at baseline and 3 months post therapy for periodontitis group
Relative Attachment level (RAL)	At baseline and 3 months	The index was recorded at only baseline for healthy and gingivitis groups and at baseline and 3 months post therapy for periodontitis group
Probing Depth (PD)	At baseline and 3 months	The index was recorded at only baseline for healthy and gingivitis groups and at baseline and 3 months post therapy for periodontitis group
Bleeding on Probing (BOP%)	At baseline and 3 months	The index was recorded at only baseline for healthy and gingivitis groups and at baseline and 3 months post therapy for periodontitis group
Plaque index (PI)	At baseline and 3 months	The index was recorded at only baseline for healthy and gingivitis groups and at baseline and 3 months post therapy for periodontitis group

Trial Locations

Locations (1)

Krishnadevaraya college of dental sciences; 🇮🇳 Bangalore, Karnataka, India