Impact of Interdental Brushing on the Evolution of Supra-gingival Bacterial Flora (BACTERIB)

Completed

• Conditions: Healthy
• Interventions: Other: Use of interdental brushes

• Registration Number: NCT03714295
• Lead Sponsor: Claude Bernard University

Brief Summary

In France, two-thirds of adults over 35 years of age have at least a periodontal disease. The etiology is the accumulation of dental plaque, especially in interdental spaces. The cleaning of interdental spaces is particularly complex. This accumulation of dental plaque is at the origin of a modification of the bacterial flora in favor of periodontopathogenic bacteria. These pathogens can induce within the periodontal tissues an immune and inflammatory reaction that cause tissue degradation. The majority of studies focus on people over 30 years of age with gingivitis or periodontitis. However, young adults, in whom the initial or even reversible stages of periodontal disease are the most frequent, should be the preferred target of preventive measures for this pathology.

The main objective of this study is to quantify, in healthy patients, the evolution of the supragingival bacterial flora, in the interdental spaces, following the daily passage of brushes with the adapted diameter and single passage. The secondary objective is to evaluate the qualitative change in the bacterial flora (proportions of periodontal bacteria / commensal bacteria) following the interdental cleaning.

Detailed Description

Not available

Study Timeline

• Study Start: 2015-04-01
• Primary Completion: 2015-04-15
• Study Completion: 2015-07-01
• Status Verified: 2018-10
• Study First Submitted: 2018-10-15
• Study First Submitted QC: 2018-10-19
• Last Update Submitted: 2018-10-19
• Study First Posted: 2018-10-22
• Last Update Posted: 2018-10-22

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 25

Inclusion Criteria

• having teeth at the 4 interdental sites studied (15-16, 25-26, 35-36, 45-46),
• reporting a minimum of two daily toothbrushes,
• having a good understanding of the French language,
• accepting the terms of the study and signing written informed consent,
• in good general health (opinion of the investigator) without significant clinical abnormality and medical history.

Exclusion Criteria

• Smoking patients (daily consumption greater than or equal to 1 cigarette),
• patients with pathologies: diabetic, haemophilia, anticoagulant, platelet antiaggregant, pregnant and lactating women,
• patients with a history of periodontal treatments,
• patients with mandibular fixed orthodontic appliances and or maxillary, masticatory coefficient less than 80%,
• patients who have taken antibiotics in the past 3 months,
• patients using interdental brushes on a regular basis (more than once a week) in addition to brushing teeth or mouthwash ,
• patients at risk for infectious endocarditis,
• patients unable to answer questions,
• non-cooperative patients,
• patients requiring geographic mobility compromising evaluation.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Use of interdental brushes	Use of interdental brushes	The group use interdental brushes one time each day and wash teeth with a classical brush two times a day
No use of interdental brushes	Use of interdental brushes	The group wash teeth with a classical brush two times a day

Primary Outcome Measures

Name	Time	Method
Enumeration of periodontal pathogens in interdental microbiota	3 months	Quantitative RT-PCR analyses for A. viscosus, S.mitis, Streptococcus sp., V. parvula, A. odontolyticus, E. corrodens, C. sputigena, C. ochracea, C. concisus, A. actino a, A. actino b, S. sobrinus.- et des bactéries pardonthopathogènes - C. gracilis, C. rectus, P. intermedia, P. nigrescens, P. micros, F. nuc, P. gingivalis, T. forsythensis, T. denticola, S. mutans, and Lactobacillus spp. in the interdental microbiota.

Secondary Outcome Measures

Name	Time	Method
Enumeration of periodontal pathogens in interdental microbiota of young adults using or not interdental brushes by real-time PCR	3 months	Quantitative RT-PCR analyses for A. viscosus, S.mitis, Streptococcus sp., V. parvula, A. odontolyticus, E. corrodens, C. sputigena, C. ochracea, C. concisus, A. actino a, A. actino b, S. sobrinus.- et des bactéries pardonthopathogènes - C. gracilis, C. rectus, P. intermedia, P. nigrescens, P. micros, F. nuc, P. gingivalis, T. forsythensis, T. denticola, S. mutans, and Lactobacillus spp. in the interdental microbiota of young adults using or not interdental brushes.

Trial Locations

Locations (1)

University Lyon; 🇫🇷 Lyon, France