Comparison of Structural and Molecular Genetic Properties Between Normal and Hypospadic Children's Prepuces
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 52
- 试验地点
- 1
- 主要终点
- Genetic analysis 2
研究概览
简要总结
The aim of this study is to compare prepuce tissue samples from healthy male children who applied for circumcision and children with isolated hypospadias. Estrogen receptor 1 (ER1), androgen receptor (AR), FGFR2, transforming growth factor beta (TGF beta) and HOXA13 gene expression in the prepuce is planned to investigate via polymerase chain reaction (PCR) method. Mucosal vascular and nerve densities, and extracellular matrix density of laminin and collagen protein is planned to investigate via immunohistochemical examination.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 年龄范围
- — 至 18 Years（Child, Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •To apply for circumcision, not to have any genitourinary abnormalities/conditions for control group.
- •To have isolated hypospadias for study group.
排除标准
- •To have genitourinary surgery before.
结局指标
主要结局
Genetic analysis 2
时间窗: 1 year
Estrogen receptor 1 (ER1) gene PCR analysis
Immunohistochemical study 2
时间窗: 1 year
Mucosal nerve density is planned to determine via PGP 9.5
Genetic analysis 5
时间窗: 1 year
HOXA13 gene PCR analysis
Histological study 2
时间窗: 1 year
Collagen is planned to determine in extracellular matrix via light microscope
Genetic analysis 1
时间窗: 1 year
Androgen receptor (AR) gene PCR analysis
Genetic analysis 3
时间窗: 1 year
FGFR2 gene PCR analysis
Genetic analysis 4
时间窗: 1 year
TGF beta gene PCR analysis
Histological study 1
时间窗: 1 year
Laminin is planned to determine in extracellular matrix via light microscope
Immunohistochemical study 1
时间窗: 1 year
Mucosal vessel density is planned to determine via CD34
次要结局
未报告次要终点