Biological Rhythms Impact of 12 Hours Shift Work
- Conditions
- Shift-Work Sleep Disorder
- Interventions
- Other: melatonin and cortisol sampling
- Registration Number
- NCT03153917
- Lead Sponsor
- Hospices Civils de Lyon
- Brief Summary
Nonstandard time-schedules work are widespread in the world of work. Shift and night work have been shown to be responsible for a desynchronization of biological rhythms, associated with melatonin secretion impairment and sleep disturbances. However, while health consequences of night and shift work are widely recognized and represent a major public health concern (High Authority of Health 2012 Recommendations and National Agency for Public Health Food, Environment and Work 2016 Report), the mechanisms by which the circadian system is affected by shift work remain poorly understood. Indeed most of the studies in the fields are cross-sectional, based on few blood or urinary samples in a single work position, in workers whose shift work patterns are often misidentified and heterogeneous. Moreover, few studies have assessed melatonin secretion alterations in relation to objective sleep measurement (actigraphy). The aim of this study is to assess the dynamics of melatonin secretion adaptation in 12-hour shift work and to correlate these data to sleep-wake cycle recording.
- Detailed Description
Not available
Recruitment & Eligibility
- Status
- COMPLETED
- Sex
- All
- Target Recruitment
- 15
- Nurses or nursing assistants working in the Sleep and Respiratory Diseases Department of the Croix-Rousse Hospital with positions in 12 hours for more than 6 months
- informed consent given by the subject
- Pregnancy or lactation <6 months
- Taking treatment modifying the secretion of melatonin (melatoninergic drugs (including antidepressants), beta-blockers)
- Refusal of participate
- Minor or major protected subject
- Not affiliated to a social security scheme
- Participant in another interventional study interfering with sleep / wake rhythm or work schedules (judgment of the investigator)
Study & Design
- Study Type
- INTERVENTIONAL
- Study Design
- SINGLE_GROUP
- Arm && Interventions
Group Intervention Description melatonin and cortisol sampling melatonin and cortisol sampling 14 healthy volunteer nurses working on 12 hours night/day schedules in the Sleep Medicine Center of Lyon.
- Primary Outcome Measures
Name Time Method total amount of melatonin excreted during the sleep period for the night shift at day 8 (night shift) The impact of 12H shift work on melatonin secretion dynamics will be assessed by comparing the total amount of melatonin excreted during the sleep period between the 12h day shift condition and the 12h night shift condition
total amount of melatonin excreted during the sleep period for the day shift. at day 2 (day shift) The impact of 12H shift work on melatonin secretion dynamics will be assessed by comparing the total amount of melatonin excreted during the sleep period between the 12h day shift condition and the 12h night shift condition
- Secondary Outcome Measures
Name Time Method Melatonin secretion dynamics in 12h day shift at day 2 (day shift) Secretion of melatonin on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Objective sleep quality 15 days Measure of intra-sleep wakefulness assessed by actigraphy
Insomnia severity at day 1 This outcome will be assessed by the Insomnia Severity Index
Chronotype at day 1 This outcome will be assessed by the Horne and Ostberg Questionary
Melatonin secretion dynamics in 12h night shift at day 8 (night shift) Secretion of melatonin on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Sleep subjective quality at day 2, 8 and 13 This outcome will be assessed by the St Mary Hospital Questionary
Light exposition 15 days This outcome will be assessed by the actigraphy
Total amount of melatonin excreted during the sleep period at rest at day 13 (rest) the total amount of melatonin excreted during the sleep period at rest will be compared to the total amount of melatonin excreted during the sleep period for the 12h day shift condition ant to the total amount of melatonin excreted during the sleep period for the 12h night shift condition
Melatonin secretion dynamics at rest at day 13 (rest) Secretion of melatonin on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Cortisol secretion dynamics in 12h day shift at day 2 (day shift) Secretion of cortisol on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Cortisol secretion dynamics in 12h night shift at day 8 (night shift) Secretion of cortisol on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Cortisol secretion dynamics at rest at day 13 (rest) Secretion of cortisol on sequential urinary specimens collected every 4 or 8 hours.
Measure of mesor (ng)Asthenia at day 1 This outcome will be assessed by the Pichot asthenia scale
Objective sleep duration 15 days duration of sleep assessed by actigraphy
Excessive daytime sleepiness at day 1 This outcome will be assessed by the Epworth sleepiness Scale
Anxiety at day 1 This outcome will be assessed by the Hospital Anxiety and Depression scale
Depression at day 1 This outcome will be assessed by the Hospital Anxiety and Depression scale
Vigilance at awakening at day 2, 8 and 13 This outcome will be assessed by the Karolinska scale
Trial Locations
- Locations (1)
Croix-Rousse Hospital
🇫🇷Lyon, France