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Sequential Regimen of Bendamustin-Debulking Followed by Obinutuzumab, Acalabrutinib and Venetoclax in Patients with Relapsed/refractory CLL

Phase 2
Completed
Conditions
Chronic Lymphoid Leukemia
Interventions
Registration Number
NCT03787264
Lead Sponsor
German CLL Study Group
Brief Summary

CLL2-BAAG is a prospective, open-label, multicenter phase-II trial to evaluate the efficacy and safety of a sequential regimen of debulking with bendamustine followed by induction and maintenance with GA101 (obinutuzumab), acalabrutinib (ACP-196) and venetoclax (ABT-199) in patients with relapsed/refractory CLL.

Detailed Description

In the CLL2-BAAG trial will be included a total of 46 patients with relapsed or refractory CLL in need of treatment.This trial will evaluate a debulking with two cycles bendamustine (only for patients with a higher tumor load), followed by an induction and a maintenance treatment with obinutuzumab, acalabrutinib and venetoclax in patients with re-lapsed/refractory CLL. The duration of maintenance treatment is depending on MRD levels. This trial combines one old (chemotherapy) and three novel, synergistic (antibody, BTK-inhibitor and Bcl-2 antagonist) principles of action in order to achieve deep and long lasting remissions with a short duration of treatment. Additionally, this trial has an extensive accompanying scientific program aiming at a better understanding of the kinetics of response and clonal evolution of CLL.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
46
Inclusion Criteria
  1. Relapsed/refractory CLL in need of treatment according to iwCLL (international workshop on CLL) criteria

    In case of a recent previous treatment, patients must have recovered from acute toxicities and treatment regimen must be stopped within the following time periods before start of the study treatment in the CLL2-BAAG trial:

    • chemotherapy ≥ 28 days
    • antibody treatment ≥ 14 days
    • kinase inhibitors, BCL2-antagonists or immuno-modulatory agents ≥ 3 days
    • corticosteroids may be applied until the start of the BAAG-regimen, these have to be reduced to an equivalent of ≤ 20mg prednisolone per day during treatment Please note: Patients with a progression during previous treatment with venetoclax, ibrutinib or another BTK inhibitor, as well as patients with a known resistance mutation (e.g. BTK-/PLCg2) are excluded from study participation. However, patients who progressed after termination of treatment with venetoclax, ibrutinib, other BTK inhibitors and/or obinutuzumab or who stopped treatment due to in-tolerance to ibrutinib are eligible for participation.
  2. Adequate renal function, as indicated by a creatinine clearance ≥30ml/min calculated according to the modified formula of Cockcroft and Gault or directly measured with 24 hr. urine collection

  3. Adequate hematologic function as indicated by a neutrophil count ≥ 1.0 x 109/L, a hemoglobin value ≥8.0 g/dL and a platelet count ≥ 25 x 109/L, unless directly attributable to the patient´s CLL (e.g. bone marrow infiltration), in this case, platelet count should be ≥ 10 × 109/L.

  4. Adequate liver function as indicated by a total bilirubin ≤2x, AST/ALT ≤2.5x the institutional ULN value, unless directly attributable to the patient's CLL or to Gilbert's Syndrome

  5. Negative serological testing for hepatitis B (HBsAg nega-tive and anti-HBc negative, patients positive for anti-HBc may be included if PCR for HBV DNA is negative and HBV-DNA PCR is performed every 4 weeks until one year after last dosage of GA101 (obinutuzumab)), negative testing for hepatitis-C RNA and negative HIV test within 6 weeks prior to registration

  6. Age ≥ 18 years

  7. ECOG (Eastern Cooperative Oncology Group) performance status 0 - 2, ECOG 3 is only permitted if related to CLL (e.g. due to anemia or severe constitutional symptoms)

  8. Life expectancy ≥ 6 months

  9. Ability and willingness to provide written informed consent and to adhere to the study visit schedule and other proto-col requirements

Exclusion Criteria
  1. (Suspicion of) transformation of CLL (i.e. Richter's trans-formation, pro-lymphocytic leukemia) or central nervous system (CNS) involvement

  2. Progression during previous treatment with venetoclax, ibrutinib or another BTK inhibitor, and/or presence of known mutations associated with resistance to therapy, e.g. Bru-ton´s Tyrosine Kinase and Phospholipase C Gamma 2 (PLCg2)

  3. Confirmed progressive multifocal leukoencephalopathy (PML)

  4. Malignancies other than CLL currently requiring systemic therapies

  5. Uncontrolled infection requiring systemic treatment

  6. Any comorbidity or organ system impairment rated with a CIRS (cumulative illness rating scale) score of 4, excluding the eyes/ears/nose/throat/larynx organ system1 or any other life-threatening illness, medical condition or organ system dysfunction that - in the investigator´s opinion - could compromise the patients safety or interfere with the absorption or metabolism of the study drugs (e.g, inability to swallow tablets or impaired resorption in the gastrointestinal tract)

  7. Significantly increased risk of bleeding according to the investigator´s evaluation, e.g. due known bleeding diathesis (e.g. von-Willebrandt´s disease or hemophilia), major surgical procedure ≤ 4 weeks or stroke/intracranial hemorrhage ≤ 6 months.

  8. Requirement of therapy with strong CYP3A4 inhibitors/inducers or anticoagulant with phenprocoumon (marcumar) or other vitamin K-antagonists

  9. Use of investigational agents ≤ 28 days prior to start of study treatment, however, kinase inhibitors, BCL2-antagonists and antibody treatment are allowed in accordance with inclusion criterion number 1 (see above).

  10. Known hypersensitivity to obinutuzumab (GA101), venetoclax (ABT-199), acalabrutinib (ACP-196) or any of the excipients Please note: Patients with a known hypersensitivity to bendamustine are allowed to participate but will not receive a debulking with bendamustine

  11. Pregnant women and nursing mothers (a negative preg-nancy test is required for all women of childbearing potential within 7 days before start of treatment)

  12. Fertile men or women of childbearing potential unless:

    • surgically sterile or ≥ 2 years after the onset of menopause, or
    • willing to use two methods of reliable contraception including one highly effective (Pearl Index <1) and one additional effective (barrier) method during study treatment and for 18 months after end of study treatment.
  13. Vaccination with a live vaccine ≤ 28 days prior to registration

  14. Legal incapacity

  15. Prisoners or subjects who are institutionalized by regula-tory or court order

  16. Persons who are in dependence to the sponsor or an investigator

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
BAAGObinutuzumabDebulking: 2 debulking cycles (q 28d) of bendamustine will be administered unless the patient has a contraindication or a debulking is not clinically indicated Induction: 6 cycles (q 28d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance: max. 8 cycles (q 84d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance treatment will be continued until (whichever occurs first): * 12 weeks (approx. 3 months) after confirmation of achievement of a CR/CRi and MRD negativity * maintenance cycle 8 * progression of CLL or start of a subsequent therapy * unacceptable toxicity
BAAGVenetoclaxDebulking: 2 debulking cycles (q 28d) of bendamustine will be administered unless the patient has a contraindication or a debulking is not clinically indicated Induction: 6 cycles (q 28d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance: max. 8 cycles (q 84d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance treatment will be continued until (whichever occurs first): * 12 weeks (approx. 3 months) after confirmation of achievement of a CR/CRi and MRD negativity * maintenance cycle 8 * progression of CLL or start of a subsequent therapy * unacceptable toxicity
BAAGAcalabrutinibDebulking: 2 debulking cycles (q 28d) of bendamustine will be administered unless the patient has a contraindication or a debulking is not clinically indicated Induction: 6 cycles (q 28d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance: max. 8 cycles (q 84d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance treatment will be continued until (whichever occurs first): * 12 weeks (approx. 3 months) after confirmation of achievement of a CR/CRi and MRD negativity * maintenance cycle 8 * progression of CLL or start of a subsequent therapy * unacceptable toxicity
BAAGBendamustineDebulking: 2 debulking cycles (q 28d) of bendamustine will be administered unless the patient has a contraindication or a debulking is not clinically indicated Induction: 6 cycles (q 28d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance: max. 8 cycles (q 84d) of Obinutuzumab + Acalabrutinib + Venetoclax Maintenance treatment will be continued until (whichever occurs first): * 12 weeks (approx. 3 months) after confirmation of achievement of a CR/CRi and MRD negativity * maintenance cycle 8 * progression of CLL or start of a subsequent therapy * unacceptable toxicity
Primary Outcome Measures
NameTimeMethod
Negativity rate of minimal residual disease (MRD) in peripheral blood (PB) measured by 4-color flow cytometryAt final restaging (RE): 12 weeks after the start of the last induction cycle

MRD negativity is defined as less than one (1) CLL-cell among 10,000 leukocytes analyzed \[0.01%\], i.e. \< 10-4. The MRD negativity rate is defined as the proportion of patients having achieved MRD negativity based on the full analysis set (FAS).

Secondary Outcome Measures
NameTimeMethod
MRD in PB measured by 4-color flow cytometry at different times: At screening, after debulking, 4-weekly during induction, at initial response assessment (after 6 induction cycles), at RE, every 12 weeks during maintenance and follow up.From date of screening until the end of follow-up, up to 40 month.

MRD negativity is defined as less than one (1) CLL-cell among 10,000 leukocytes analyzed \[0.01%\], i.e. \< 10-4. MRD values will be categorized into negative (\<10-4) and positive (≥10-4)

Overall response rate (ORR)At final restaging (RE): 12 weeks after the start of the last induction cycle

Proportion of patients having achieved a complete response (CR), a CR with incomplete recovery of the bone marrow (CRi), or a partial response(PR) as best response.

CR / CRi rateAt final restaging (RE): 12 weeks after the start of the last induction cycle

Proportion of patients having achieved a CR or CRi as best response

Safety: Adverse events (AE), serious adverse events (SAE) and adverse events of particular interest (AEPI)up to 40 months after first dose of study drug

Type, frequency, and severity of AEs, SAEs and AESIs and their relationship to study treatment.

Trial Locations

Locations (17)

Gemeinschaftspraxis Hämatologie Onkologie

🇩🇪

Dresden, Germany

Universitatsklinik Carl Gustav Carus

🇩🇪

Dresden, Germany

Praxis fuer Haematologie und Onkologie

🇩🇪

Koblenz, Germany

Krankenhaus Muenchen-Schwabing

🇩🇪

Munich, Germany

Ludwig-Maximilians-Universitaet Muenchen

🇩🇪

München, Germany

Praxis Dr. Uhlig

🇩🇪

Naunhof, Germany

Universitätsklinik Rostock

🇩🇪

Rostock, Germany

Helios Klinikum Erfurt

🇩🇪

Erfurt, Germany

Universitaetsklinikum Heidelberg

🇩🇪

Heidelberg, Germany

Universitaetsklinik Tuebingen

🇩🇪

Tübingen, Germany

Universitaetsklinikum Jena

🇩🇪

Jena, Germany

Klinikum Leverkusen GmbH

🇩🇪

Leverkusen, Germany

Universitätsklinikum Ulm

🇩🇪

Ulm, Germany

Gemeinschaftspraxis Haemato/ Onkologie Lebach

🇩🇪

Lebach, Germany

ZAHO-Rheinland

🇩🇪

Siegburg, Germany

Gemeinschaftspraxis Mohm/Prange-Krex

🇩🇪

Dresden, Germany

Universitätsklinik Köln

🇩🇪

Köln, Germany

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