Effect of root planning in microbiological, White blood cells, immunoglobulins and pro and anti inflamatory cytokines dosage in blood and saliva from periodontitis and type 2 diabet persons

Not Applicable

• Conditions: Chronic periodontitis.Diabetes type 2; C07.465.714.533.324; C18.452.394.750.149

• Registration Number: RBR-6268zz
• Lead Sponsor: niversidade de Brasília

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Results First Posted: 1900-01-01
• Study Start: 2016-03-05
• Last Update Posted: 29 May 2023

Recruitment & Eligibility

• Status: Recruitment completed
• Sex: Not specified
• Target Recruitment: Not specified

Inclusion Criteria

Minimum of 15 teeth; least 30 percent of the sites with periodontal probing depth greater than or equal to 4 mm; at least two teeth with greater insertion loss than 3 mm; Age greater than or equal to 30 years. The control group will consist of individuals aged greater than or equal to 30 years; systemically healthy; Clinical and radiographic periodontal health; Minimum 20 teeth with less clinical probing depth or equal to 3 mm; lower insertion level at 3 mm; less than 10% of sites with bleeding on probing

Exclusion Criteria

Periodontal treatment in the last six months; Continued use of medications such as antibiotics, immunomodulators or anti-inflammatory drugs in the last three months; Systemic diseases that interfere with periodontal condition, except for diabetes for the test group (HIV, immune disorders, morbid obesity (BMI 40 kg / m2) or underweight (BMI less malnourished 18.5 kg / m2), etc.) ; Pregnant or lactating; smoking; Under age 30; Indigenous groups

Study & Design

• Study Type: Intervention
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Alteration of cell profiles evaluated in patients with type 2 diabetes and chronic periodontitis, by flow cytometry, from the finding of a change of at least 5% in the pre and post-intervention measurements.

Secondary Outcome Measures

Name	Time	Method
Alteration of systemic mediators evaluated in patients with type 2 diabetes and chronic periodontitis, using the enzyme linked immunosorbent assay (ELISA), from the finding of a change of at least 5% in the pre and post intervention measurements of cytokines pro and anti-inflammatory.;Alteration of systemic mediators evaluated in patients with type 2 diabetes and chronic periodontitis, using the dotenzyme immunoassay technique (dot-ELISA), from the finding of a change of at least 5% in pre- and post-intervention measurements of immunoglobulins A , G and M.;Changing the microbiological profile evaluated in patients with type 2 diabetes and chronic periodontitis, using the polymerase chain reaction (PCR), from the finding of a change of at least 5% in pre measurements and post intervention of 8 main periodontopathogens.