The Impact of Stress and Caregiver Sensitivity on Infant Cellular Aging in a Population of Under-Resourced Families: A Randomized Controlled Trial

简要总结

详细描述

Early life adversity (ELA) is a salient risk factor for later-life morbidity and early mortality. During the first years of life, children are particularly vulnerable to adverse events. Notably, these adversities are disproportionately placed on families of color and can lead to health disparities. Cellular aging is a potential mechanism by which ELA confers lifelong risks. Interventions implemented during sensitive developmental periods early in life may yield a higher efficacy and reduce health disparities. The aim of the proposed randomized controlled trial (RCT) is to test if an evidence-based home visiting model, Promoting First Relationships® (PFR), that improves caregiver sensitive and responsive care, when embedded within a primary care setting, will protect young infants from the effect of stress on accelerated cellular aging. Telomeres, the structures at the end of chromosomes, are considered a marker for cellular aging. Telomeres shorten with each cell division and this can lead to cellular senescence, one of the key hallmarks of aging. Shorter telomere length (TL) is associated with poorer physical and mental health outcomes. In addition to TL, "epigenetic clocks", which are composed of dozens or hundreds of methylation marks, predict mortality and are linked with disability and disease processes. There is preliminary evidence that epigenetic age (EpiAge) is accelerated by perinatal risk exposures and lifetime adversity, and that stress-related mechanisms forecast accelerated EpiAge. The literature has shown that TL and EpiAge are malleable and receptive to environmental inputs, interventions that focus on family support and a healthy lifestyle, as well as parental sensitivity, which operates as a protective factor. The investigators have an incredible opportunity to advance the field to understand the impact of past and present stressors, caregiver sensitive and responsive care, on infant cellular aging (including both TL and EpiAge clocks), in a sample of under-resourced families receiving PFR through their primary care setting. PFR is a 10-week home visiting model that has, in five prior RCTs, consistently improved caregiver sensitivity and responsive care, as well as parental knowledge of children's social-emotional needs. PFR has shown improvements at the biological level in children through Respiratory Sinus Arrhythmia, a marker of emotional regulation, and stimulated cortisol responses. This study will expand upon these findings and will be the first to evaluate PFR, delivered in the context of primary care (PFR-PC), on children's cellular aging. The setting for this study is unique. WakeMed Primary Care in North Carolina is a busy pediatric practice that utilizes an integrated care model to serve a high-need patient population. Their aim is to reduce disparities through services embedded within primary care. WakeMed's pediatric practice serves nearly 7,000 families in one of the highest-need zip codes in the state. Of their highly diverse patients, 90% receive Medicaid. Embedding PFR within a primary care setting is an innovative strategy to facilitate an integrated care model and to enhance resilency in minoritized lower income families. In this RCT, we will recruit a low-income, community sample of 250 Spanish- and English-Speaking mothers and their infants receiving primary care from WakeMed. Mothers will be randomized to Usual Care (UC) or to PFR in Primary Care (PFR-PC) 10-week home visiting with follow-up content during two well-child visits. We will collect dried blood spots from mothers and children to evaluate TL and various EpiAge clocks. We will also advance the literature by accounting for important heritability factors (i.e., maternal TL at baseline) and intergenerational controls (i.e., maternal Adverse Childhood Experiences (ACEs) and paternal age at conception). The investigators will measure mothers' TL at baseline and child TL and EpiAge clocks at baseline and one-year post-baseline. Aim 1: Evaluate three under-studied heritability and intergenerational predictors of cellular aging: Test whether 1) maternal TL, 2) maternal ACEs, and 3) paternal age at conception predict child baseline TL. It is hypothesize that heritability and intergenerational measures will predict child baseline TL. The investigators will further evaluate the effect of maternal ACEs on child EpiAge clocks at baseline. Aim 2: Evaluate the impact of adversity on change in cellular aging. We will test if current adversity (caregiver depression, discrimination, violence, and difficult life events) predicts child cellular aging (TL and EpiAge clocks) between baseline and 12 months post-enrollment, controlling for heritability and intergenerational effects (Aim 1) and PFR-PC. It is hypothesize that children exposed to greater concurrent adversity will evidence accelerated cellular aging compared with children with less adversity. Aim 3: Test whether PFR-PC reduces accelerated cellular aging. Test PFR-PC on change in TL and EpiAge among children in a population with high health disparities one-year post-intervention. It is hypothesize that 1) children in the PFR-PC treatment group will have significantly longer TL and younger EpiAge than those in the UC control group 1-year post enrollment. It is also hypothesize that 2) treatment difference in TL/EpiAge will be mediated by maternal sensitivity using standard mediation and that PFR will improve mother sensitivity and child behavior. Exploratory aim. Evaluate PFR-PC implementation (dosage, quality, acceptability, satisfaction, and provider fidelity). Based on prior studies, it is hypothesize that PFR will demonstrate successful implementation (dosage, quality, acceptability, satisfaction, and provider fidelity).

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次要结局

• Child Behavior Problems change(Baseline (Time 1), 6- months post baseline (Time 2), 12-months post-baseline (Time 3))