MENSTRUAL CYCLE AND CHANGES IN HEART

Not Applicable

Recruitment & Eligibility

Inclusion Criteria

1. Subject who gives written inform consent

2. Subjects should belong to the specified age range (18-45 years).

3. They should have regular menstrual cycles of 27 to 35 days as established by history, for at least the past 6 months.

Exclusion Criteria

1. Participant refusal to participate in study

2. Female subjects below 18 years and above 45years

3. Subjects having irregular menstrual cycle. (anovulatory cycle)

4. Subjects taking any medication or hormonal preparations that could alter the menstrual hormonal milieu.

5. Subjects having any physical illness like hypertension, cardiac arrhythmia, TB, diabetes or any other endocrinological disorders.

6. Smokers, alcoholics or those with history of substance abuse.

7. Athletes and those involved in excessive physical activity.

8. Subjects having any disorder which can interfere with the autonomic responses.

Primary Outcome Measures

Name	Time	Method
Sympathetic function test <br/ ><br>Resting Systolic and Diastolic BP <br/ ><br>Blood pressure response to standing <br/ ><br>Blood pressure response to sustained handgrip <br/ ><br>Parasympathetic function test <br/ ><br>Resting heart rate <br/ ><br>Heart rate variation during deep breathing <br/ ><br>E/I ratio <br/ ><br>Immediate response to standing 30:15 ratio <br/ ><br>Valsalva ratio <br/ ><br> <br/ ><br>Heart Rate Variability parameters <br/ ><br>Time Domain Parameters <br/ ><br>SDNN <br/ ><br>RMSSD <br/ ><br>SDNN index <br/ ><br>SDSD <br/ ><br>NN50 count <br/ ><br>pNN50 <br/ ><br> <br/ ><br>Frequency Domain Parameters <br/ ><br>HF <br/ ><br>LF <br/ ><br>VLF <br/ ><br>LF (nor) <br/ ><br>HF (nor) <br/ ><br>HF/LF ratio <br/ ><br> <br/ ><br>Timepoint: follicular phase - 9-12th day of menstrual cycle <br/ ><br>secretory phase- 19- 22nd day of menstrual cycle

Secondary Outcome Measures

Name	Time	Method
Sympathetic function test <br/ ><br>Resting Systolic and Diastolic BP <br/ ><br>Blood pressure response to standing <br/ ><br>Blood pressure response to sustained handgrip <br/ ><br>Parasympathetic function test <br/ ><br>Resting heart rate <br/ ><br>Heart rate variation during deep breathing <br/ ><br>E/I ratio <br/ ><br>Immediate response to standing 30:15 ratio <br/ ><br>Valsalva ratio <br/ ><br> <br/ ><br>Heart Rate Variability parameters <br/ ><br>Time Domain Parameters <br/ ><br>SDNN <br/ ><br>RMSSD <br/ ><br>SDNN index <br/ ><br>SDSD <br/ ><br>NN50 count <br/ ><br>pNN50 <br/ ><br> <br/ ><br>Frequency Domain Parameters <br/ ><br>HF <br/ ><br>LF <br/ ><br>VLF <br/ ><br>LF (nor) <br/ ><br>HF (nor) <br/ ><br>HF/LF ratio <br/ ><br>Timepoint: follicular phase - 9-12th day of menstrual cycle <br/ ><br>secretory phase- 19- 22nd day of menstrual cycle