HIV (Human Immunodeficiency Virus) Subtype and ARV Response in African Children

Study Setting The study was carried out at the Paediatric Infectious Disease Clinic (PIDC) now Baylor College of Medicine Bristol Myers Squibb Children's Clinical Centre of Excellence at Mulago Hospital. The PIDC is an HIV outpatient clinic operating 5 days a week with three paediatric clinics, one adolescent and one family-based clinic. The clinic provides care to one hundred and eighty children with HIV/AIDS daily. Approximately 50 children per month qualify for antiretroviral therapy based upon clinical and laboratory parameters.

The Makerere University Faculty of Medicine, Research Committee and Ethics Committee, the Uganda National Council of Science and Technology and IRB boards of Baylor College of Medicine and the University of Wisconsin Hospital and Clinics approved this study. Informed consent and assent (when applicable) was obtained from the primary caregiver and/or child before enrollment.

Study Participants and Enrollment Starting in 2007 children between the ages of 6 weeks and 18 years having confirmed HIV infection and receiving no ART were consecutively enrolled into this observational cohort study following consent and/or assent (where applicable). Children were excluded from participation if they were enrolled in another cohort study, or had psychosocial issues precluding participation (no parent or guardian willing to promote ART adherence or clinic HIV counselor evaluation of non adherence). The enrollment goal for this study was approximately100 children recruited over a period of 8 months.

Initiation of ART and Study Design Children confirmed to be HIV infected were screened with clinical history and examination by the study physician to determine eligibility. One hundred and twenty two children were recruited and 111 were enrolled into this prospective cohort study. Once enrolled a decision to start ART was based upon guidelines set forth by WHO (2006 guidelines with addendum in 2008)/UNAIDS recommendations, the Ugandan National Policy on ART and laboratory parameters (described below). ART treatment decisions were made at the discretion of the treating physician. Adherence monitoring was done by means of self-reporting and pill counts during patient visits to the clinic. Antiretroviral (ARV) medications used in the study included: zidovudine (AZT), lamivudine (3TC), abacavir (ABC), stavudine (d4T), nevirapine (NVP), efavirenz (EFV), didanosine (ddI), tenofovir/emtricitabine (Truvada), lopinavir/ritonavir (Aluvia/Kaletra). History of peripartum NVP use by the child and/or mother was obtained through interview.

Clinical assessment with disease staging (both WHO and CDC) and baseline laboratory investigations which included complete blood count (CBC), renal and liver function tests (RFT and LFT, respectively), CD4%/absolute count, HIV-1 RNA level and genotype were done prior to the patient starting ART. All blood samples were labeled with a study number that was used for participant identification. Clinical assessment was done at every patient visit. VL monitoring was done every three months during the first year of ART follow up and every 6 months thereafter to the end of the study period. CD4%/absolute count and safety laboratory evaluations were done every 6 months. Genotype evaluation was done prior to starting therapy and when virological failure was documented. Virologic failure was defined as two consecutive (for our clinic one month apart) HIV-1 RNA levels >400 copies/ml occurring at any time during the study period in a child who previously was suppressed or had a 1-2 log10 response to ART; a persistent viral load elevation (>400 copies/ml) during the first 3 months of ART; or a viral load >1000 copies/ml on the last blood drawn from a child at the end of the study period, before death, or at withdrawal or lost to follow-up. Initial virologic response was defined as a decrease in the HIV-1 RNA level (viral load) of at least 1 log from baseline after 4-8 weeks of ART. A full virologic response was defined as an HIV-1 RNA level <400 copies/ml (undetectable) by week 24 and/or 48 after starting ART.