Impact of Probiotics in Modulation of Intestinal Microbiota of Patients Undergoing a Resection Colonic
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 33
- 试验地点
- 2
- 主要终点
- Difference in gene expression of citokynes
研究概览
简要总结
The investigators would study about impact of the administration of probiotics in the intestinal mucosa of patients undergoing resection colic, by evaluating cytokine profile by quantitative real time PCR. The investigators believe that patients who use probiotic preoperative would provide cytokine profile less inflammatory than those of the control group.
详细描述
Samples of colorectal cancer-free mucosa derived from patients who underwent surgical resection were investigated. Quantitative real-time polymerase chain reaction (RT-qPCR) was used to determine expression levels of the following genes: IL10, IL1B, IL23A, TNF, IL12B, INFG, IL17A.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Parallel
- 主要目的
- Health Services Research
- 盲法
- None
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •if he or she were older than 18 years
- •intended to undergo elective CRC resection at UFMG Hospital
排除标准
- •Patients were excluded if they were not able to receive the probiotics seven days before the operation
- •if colon resection was not performed due to changes in operation strategy, or if they discontinued probiotic use on their own or
- •if they removed their consent.
结局指标
主要结局
Difference in gene expression of citokynes
时间窗: 15 days
Colonic mucosal specimens were analyzed to determine the mucosal expression profiles of IL1B, IL12B, IL10, IL23A, INFG, IL17A and TNF.
次要结局
- Postoperative complications(30 days)
研究者
Maria Isabel Toulson Davisson Correia
PhD.
Federal University of Minas Gerais