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Effects of Cyproterone Compound-spironolactone, Metformin and Pioglitazone on Inflammatory Markers in PCOS

Early Phase 1
Completed
Conditions
Polycystic Ovary Syndrome
Interventions
Registration Number
NCT02689843
Lead Sponsor
Shiraz University of Medical Sciences
Brief Summary

The aim of this study is to evaluate the effects of three-month course of treatment modalities (Cyproterone compound-Spironolactone, Metformin and Pioglitazone) in patients with polycystic ovary syndrome (PCOS) on markers of inflammation \[serum complement, homocysteine and high sensitive C-reactive protein (hs-CRP)\] levels.

Detailed Description

Polycystic ovary syndrome (PCOS) is estimated to affect up to10% of women of reproductive age, making it one of the most common endocrine disorders in this population. PCOS is associated with a broad range of adverse sequel, including hypertension, dyslipidemia, insulin resistance, hyperandrogenaemia, gestational and type 2 diabetes,which ultimately increase the cardiovascular morbidity in these patients. Also PCOS is increasingly recognized as a component of the metabolic syndrome. Management depends on symptoms or the source of androgen excess. Several treatment options are available, which allows for an individualized approach. Spironolactone is the safest potent available antiandrogen. It is effective in lowering the hirsutism score by approximately one third, although considerable individual variations exist. Other antiandrogens used to treat hirsutism and hirsutism equivalents include cyproterone acetate that has weak antiglucocorticoid effects. Metformin and thiazolidinediones, are promising adjuncts for treating PCOS. Although both of them increase insulin sensitivity, but their mechanism of action differ.

Serum complement, homocysteine and C-reactive protein (CRP) levels have been reported to be linked with insulin resistance.

The investigators want to measure serum complement, homocysteine and hs-CRP levels in patients with PCOS before and after three-month course of treatment with Cyproterone compound-Spironolactone (CC-S), metformin (M) and pioglitazone (P).

Recruitment & Eligibility

Status
COMPLETED
Sex
Female
Target Recruitment
90
Inclusion Criteria
  • Age 18-35 years

  • Diagnosis of Polycystic Ovary Syndrome (PCOS) according to Rotterdam criteria 2003 (two out of three required):

    1. Oligomenorrhea or anovulation
    2. Clinical and/or biochemical signs of hyperandrogenism
    3. Polycystic ovaries (by ultrasound)
Exclusion Criteria
  • Smoking
  • Pregnancy
  • Diabetes mellitus
  • Renal failure (serum creatinine >1.5)
  • Congenital adrenal hyperplasia
  • Hyper or hypothyroidism
  • Sex hormone therapy or antiandrogen therapy during the last three months
  • Unexplained serum alanin aminotransferase (ALT) elevation more than 2.5 times above normal range
  • Any systemic or febrile illnesses
  • Use of glucocorticoid or anti-inflammatory drugs during the last three months
  • Androgen secreting tumor
  • Malignancy

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Cyproterone compound + SpironolactoneCyproterone compound + SpironolactoneCyproterone compound (Cyproterone acetate 2mg+Ethinyl estradiol 35 mcg) once daily + Spironolactone 50 mg twice daily
MetforminMetforminMetformin 1500 mg daily
PioglitazonePioglitazonePioglitazone 30 mg daily
Primary Outcome Measures
NameTimeMethod
Serum C3 level3 months

Serum concentration of a component of complement cascade: C3

Serum high-sensitive CRP (C-reactive protein)3 months

Serum concentration of high-sensitive C-reactive protein (hs-CRP)

Serum C4 level3 months

Serum concentration of a component of complement cascade: C4

Secondary Outcome Measures
NameTimeMethod
Serum free Testosterone level3 months

Serum concentration of free Testosterone

Serum Dehydroepiandrosterone sulfate (DHEAS) level3 months

Serum concentration of Dehydroepiandrosterone sulfate

Serum homocysteine level3 months

Serum concentration of homocysteine

Serum total Testosterone level3 months

Serum concentration of total Testosterone

Serum follicle stimulating hormone (FSH) level3 months

Serum concentration of follicle stimulating hormone

Serum luteinizing hormone (LH) level3 months

Serum concentration of luteinizing hormone

Fasting Blood Sugar (FBS)3 months

Fasting Blood Sugar

Fasting Serum Insulin level3 months

Serum concentration of insulin

Number of patients with adverse events3 months

Number of patients with adverse events

Trial Locations

Locations (1)

Shahid Motahhari Clinic, Shiraz University of Medical Sciences

🇮🇷

Shiraz, Fars, Iran, Islamic Republic of

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