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Sentinel Node Mapping With Indocyanine Green in Colon Cancer: a Feasibility Trial and a Descriptive Serie.

Not Applicable
Completed
Conditions
Colon Cancer
Lymphatic Metastasis
Interventions
Procedure: Sentinel node mapping
Registration Number
NCT02167087
Lead Sponsor
Ismail Gögenür
Brief Summary

This study is a clinical feasibility trial that will contribute to the clarification of whether sentinel node mapping with indocyanine green (ICG) provides a better basis for staging of colorectal cancer.

Detailed Description

The patients assigned for colon cancer will be operated as planned using laparoscopic techniques and the operation follows the normal procedure, in addition a sentinel node mapping is performed.

Indocyanine green is injected around the tumor by laparoscopic technique, the fluorescence is controlled and after 20 minutes the surgeon looks for sentinel nodes in a standardized manner. In the meantime, the surgeon proceeds with the operation as usual, except that the mesocolon can be resected only after sentinel node mapping has been performed.

After surgery the surgeon and the pathologist inspect the resected colon together and agree on D1, D2 and D3 margins. Any marked sentinel node(s) is verified and the location is checked on the colon diagram on the case report form. In addition, an ex vivo sentinel node mapping will be performed by the pathologist right after the surgery has ended. This is done to investigate if it is the same lymph nodes there will be identified by ex vivo as by intraoperative (in vivo) sentinel node mapping.

The lymph nodes in the resected colon and mesocolon are all analysed. Sentinel nodes and an equal number of randomly chosen non-sentinel nodes will be analysed further with additional sections and immune histochemistry.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
30
Inclusion Criteria
  • Male and female patients above 18 years of age scheduled for laparoscopic colon cancer surgery in the department of Surgery, Herlev Hospital or Roskilde Hospital.
Exclusion Criteria
  • iodine allergy
  • Poor kidney function (as it imply an increased risk of allergic reaction), estimated glomerular filtration rate should be above 40 ml/min.
  • Liver cirrhosis, Child-Pugh-score B and C [14]
  • Pregnancy and lactation
  • Previous anaphylactic reaction to a dye injection
  • Previous abdominal surgery

Study & Design

Study Type
INTERVENTIONAL
Study Design
SINGLE_GROUP
Arm && Interventions
GroupInterventionDescription
Sentinel node mappingSentinel node mappingSentinel node mapping with indocyanine green injected orally and anally to the tumor.
Primary Outcome Measures
NameTimeMethod
Number of procedures with successful identification of sentinel nodesday 1

Sentinel nodes is defined as the lymph node, first in line, draining from the cancer.

Secondary Outcome Measures
NameTimeMethod
Number of sentinel nodes identified during surgery and their localizationday 1
Number of cases where the sentinel node procedure has changed the clinical course of the patientweek 1

if staging and oncological treatment are changed because of the procedure

Total number of lymph nodesweek 1
Number of sentinel nodes identified by ex vivo sentinel node mappingweek 1
Malignant status of the sentinel node(s)week 1
Malignant status of the lymph nodesweek 1
Number of procedures where the procedure is stopped due to technical reasonsday 1
Number of cases with negative sentinel node, but positive non-sentinel nodeweek 1
Localization of sentinel nodes with metastasisweek 1
Localization of lymph nodes with metastasis according to D1, D2 and D3 resection lines and if they are within 1 cm from a sentinel nodeweek 1
Registration of possible side effects occurring due to the use of indocyanine greenday 1
Number of sentinel nodes identified by only one of the sentinel node mapping techniquesweek 1
Localization of the identified sentinel nodes, and whether or not they are included within the normal resection linesweek 1

Trial Locations

Locations (1)

Herlev Hospital

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Herlev, Denmark

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