Antioxidant Profile in Unstimulated Saliva in Patients With Systemic Sclerosis and Periodontitis
试验速览
- 阶段
- 不适用
- 状态
- 进行中（未招募）
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Clinical attachment level (CAL)
研究概览
简要总结
The aim of the research will be to compare periodontal status and level of antioxidants in unstimulated saliva of systemic sclerosis patients with periodontitis and systemically healthy periodontitis patients.
Twenty systemic sclerosis patients with periodontitis (SS group) and twenty systemically healthy periodontitis patients (P group) will be enrolled in the present study. The results may indicate higher periodontal destruction and antioxidant perturbations in unstimulated saliva of systemic sclerosis patients with periodontitis compared to systemically healthy periodontitis patients.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •established diagnoses of systemic sclerosis and periodontitis (study group),
- •presence of minimum 12 teeth
排除标准
- •concurrent inflammatory mediated rheumatic diseases
- •pregnancy or lactation
- •active cigarette smokers
- •a history of periodontal, antibiotics or non-steroidal drug therapy in the past 6 months.
结局指标
主要结局
Clinical attachment level (CAL)
时间窗: one day
Measuring the CAL by periodontal probe, measured in mm
Probing depth (PD)
时间窗: one day
Measuring the PD by periodontal probe, measured in mm
Gingival margin level (GML)
时间窗: one day
Measuring the GML by periodontal probe, measured in mm
The degree of gingival inflammation
时间窗: one day
The degree of gingival inflammation was assessed using the Loe and Silness (LSGI) gingival index.
plaque index (PI)
时间窗: one day
Evaluation of oral hygiene status was recorded with a Silness Loe plaque index (PI).
The concentration of vascular endothelial growth factor (VEGF)
时间窗: baseline and two months after periodontal treatment
The level of VEGF were determined using commercial ELISA (enzyme-linked immunosorbent assay) kits.
The concentration of transforming growth factor beta (TGF-β)
时间窗: baseline and two months after periodontal treatment
The level of TGF-β in gingival crevicular fluid was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer\'s instructions.
The concentration of interleukin (IL)-17
时间窗: baseline and two months after periodontal treatment
interleukin (IL)-17 was determined using commercial ELISA (enzyme-linked immunosorbent assay) kits at the Implant Research Center, School of Dental Medicine, University of Belgrade, following the manufacturer\'s instructions.
The concentration of glutathion peroxidase (GPX)
时间窗: one day
Activity level of GPX, determined using the colorimetric method and commercial kits.
The concentration of uric acid (UA)
时间窗: one day
Activity level of UA, determined using the colorimetric method and commercial kits.
The concentration of superoxide dismutase (SOD)
时间窗: one day
Activity level of SOD, determined using the colorimetric method and commercial kits.
The concentration of tumor necrosis factor alpha
时间窗: baseline and two months after periodontal treatment
The concentration of C reactive protein
时间窗: baseline and two months after periodontal treatment
Erythrocyte sedimentation rate (ESR)
时间窗: baseline and two months after periodontal treatment
次要结局
未报告次要终点
研究者
Natasa Nikolic Jakoba
Clinical Professor
University of Belgrade