Amino Acid Metabolism Before and After Bariatric Surgery

Recruiting

• Conditions: Obesity, Morbid

• Registration Number: NCT07008352
• Lead Sponsor: Harran University

Brief Summary

This observational study aims to investigate the changes in serum amino acid metabolism before and after bariatric surgery in obese individuals. Blood samples will be collected from 50 patients at baseline (preoperative), and at 1 and 3 months postoperatively. An additional 50 healthy individuals will serve as the control group. Amino acid profiling will be conducted using LC-MS/MS. The study seeks to determine how bariatric surgery affects metabolic pathways associated with amino acid regulation.

Detailed Description

Obesity is a major global health problem that significantly increases the risk of metabolic disorders. Bariatric surgery is one of the most effective interventions for sustained weight loss in morbidly obese patients. However, the metabolic effects of such surgery on amino acid profiles are not fully understood.

In this prospective observational study, a total of 100 participants will be enrolled: 50 patients undergoing bariatric surgery and 50 healthy controls. Serum samples will be collected from the patient group preoperatively, and at 1 and 3 months after surgery. The control group will provide one-time blood samples.

All serum samples will be centrifuged and stored at -80°C until analysis. Free amino acid profiles will be determined using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The primary outcome is to assess the change in amino acid concentrations over time in surgical patients, and to compare them with healthy controls. Secondary analyses will explore correlations between amino acid levels and body mass index (BMI). Data analysis will be performed using SPSS and MetaboAnalyst 5.0.

This study will help clarify how bariatric surgery influences amino acid metabolism and may offer insights into the metabolic mechanisms underlying postoperative recovery and weight regulation.

Study Timeline

• Study First Submitted: 2025-05-29
• Study First Submitted QC: 2025-05-29
• Status Verified: 2025-06
• Study First Posted: 2025-06-06
• Last Update Submitted: 2025-06-10
• Study Start: 2025-06-11
• Last Update Posted: 2025-06-11
• Primary Completion: 2025-09-10
• Study Completion: 2025-11-10

Recruitment & Eligibility

• Status: RECRUITING
• Sex: All
• Target Recruitment: 100

Inclusion Criteria

• Age ≥18 years
• Diagnosed with obesity and scheduled for bariatric surgery (study group)
• Healthy individuals with normal BMI (control group)

Exclusion Criteria

• Age <18 years
• History of metabolic or chronic systemic disease
• Pregnancy or lactation

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Change in serum free amino acid levels after bariatric surgery	Preoperative (baseline), 1 month postoperative, 3 months postoperative	Amino acid concentrations will be measured in serum samples collected from bariatric surgery patients at baseline, 1 month, and 3 months postoperatively using LC-MS/MS. The outcome will assess time-dependent changes in the amino acid profile within the same individuals.

Secondary Outcome Measures

Name	Time	Method
Correlation between serum amino acid levels and body mass index (BMI)	Preoperative (baseline), 1 month postoperative, 3 months postoperative	This outcome will evaluate the relationship between changes in serum amino acid concentrations and BMI values measured at baseline, 1 month, and 3 months after bariatric surgery. Pearson or Spearman correlation analyses will be performed.

Trial Locations

Locations (1)

Veli Fahri Pehlivan; 🇹🇷 Şanlıurfa, Turkey