Diagnostic Value of Cell Block Immunohistochemistry in Effusion Cytology
试验速览
- 阶段
- 不适用
- 入组人数
- 100
- 试验地点
- 1
- 主要终点
- Diagnostic performance of MOC-31
研究概览
简要总结
Cytology is usually the first step in investigating serous effusions, to either detect or exclude an underlying malignancy. This study will try to answer the need for improved diagnostic yield of cytologic examination by cell block technique and immunohistochemical testing of three markers which are EZH2, Claudin-4 and MOC-31.
详细描述
This is a prospective study that will be conducted at the pathology lab of Assiut University Hospital. The study will include effusion specimens (pleural, peritoneal & pericardial) and the aspirated fluid will be examined for physical characters e.g. color and appearance.
The sample will be divided into 2 equal parts: one for conventional smear preparation, and the other for cell block. A minimum of 2 thin smears will be prepared and stained with Papanicolou stain.
Cell block sections of 4-6 μ thickness will be prepared and stained with the hematoxylin and eosin stain (H&E).
For immunohistochemistry, cell blocks will be used. Expression of claudin 4, EZH2, and MOC-31 will be evaluated in benign and malignant cells by using immunoperoxidase technique.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Cross Sectional
入排标准
- 性别
- All
- 接受健康志愿者
- 否
入选标准
- •all cases with serous effusion of unknown aetiology including ascites, pleural or pericardial effusions.
排除标准
- •No exclusion criteria from the histopathologist perspective, i.e. all cases that undergone diagnostic aspiration are eligible for the study.
结局指标
主要结局
Diagnostic performance of MOC-31
时间窗: within 48 hours
sensitivity and specificity of MOC-31 for diagnosis of malignancy
Diagnostic performance of claudin4
时间窗: within 48 hours
sensitivity and specificity of claudin-4 for diagnosis of malignancy
Diagnostic performance of EZH2
时间窗: within 48 hours
sensitivity and specificity of EZH2 for diagnosis of malignancy
次要结局
- Prevalence of malignancy among cases presenting with effusion(one year)
研究者
Aliaa Elhosainy Mohammad Mahmoud
Associate Lecturer
Assiut University