Using the SpiderMass(TM) for in Vivo and Real Time Mass Spectrometry Analysis of Subject's Epidermis Suffering From Chronic Inflammatory Dermatosis Compared to Control Groups
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Acne
- Sponsor
- Pierre Fabre Dermo Cosmetique
- Enrollment
- 210
- Locations
- 1
- Primary Endpoint
- Signals and molecular profiles (including lipid) of the epidermis
- Status
- Completed
- Last Updated
- last year
Overview
Brief Summary
The physiological states of the skin are characterized by a certain homeostasis linked to the balance of the metabolic pathways. When these pathways are deregulated, the proteic, lipidic and metabolic is affected. It is thus possible to follow a change in the state of the skin by looking at change in the associated molecular profile.
The PRISM laboratory (INSERM U1192) in Lille has developed an innovative system laser called SpiderMass composed of 4 parts:
- A laser used for the micro-sampling of material in vivo,
- A transport transfer line of the ablated particles,
- A mass spectrometer that analyzes them in real time and generates the molecular profiles of the epidermis,
- A data analysis procedure.
The SpiderMass(TM) is of great interest for the study of the skin because it allows non-invasive vivo characterization, and therefore without biopsy or sample preparation. In addition, it will complement techniques already used in the research center such as FTIR spectroscopy. Indeed, in acne studies the FTIR allows to obtain only the Fatty Acid Triglycerid ratio while the SpiderMass permits to detail these lipid classes by each observed molecule on the surface of the skin and follow their evolution.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Signals and molecular profiles (including lipid) of the epidermis
Time Frame: baseline
Defined by characteristic peak mass intensities - SpiderMass
Cutaneous hydratation for atopic dermatitis subgroup and its associated control
Time Frame: baseline
By hydratation index - Corneometer
Global lipid quantity measured on face acnea, scalp seborrheic dermatitis, face rosacea (papulotelangiectasic stage of erythrocouperosis) and corresponding control volunteers
Time Frame: baseline
Global sebum level measurement expressed in arbitrary index with a Sebumeter SM815 system (Courage+Khazaka electronic GmbH, Köln. Germany)
Molecular profiles (including lipid) of the epidermis according to sampling method and the measurements of the signals obtained method
Time Frame: baseline
* sampling method - absorbant paper, in vivo and / or by scotch * measurements of the signals obtained -in vivo or FTIR
State of the cutaneous barrier for atopic dermatitis and corresponding control volunteers
Time Frame: baseline
Rate of the Trans Epithelial Water Loss (TEWL) by arbitrary units with an Aquaflux system (Biox Systems Ltd, London. UK)
Impact of several factors (dermatosis, area, gender, age) on the molecular profiles of the epidermis
Time Frame: baseline
Characteristic peak mass intensities - SpiderMass
Skin state evaluation on the lesional and perilesional areas
Time Frame: baseline
Illustrative descriptions of the skin state with photographs, taken by an iPhone 6S (Apple, Cupertino. Califronia, USA) dedicated to the study