Protein Ingestion Before Training Low

Not Applicable

Completed

• Conditions: Protein Metabolism
• Interventions: Other: Protein ingestion; Other: Non-caloric placebo ingestion

• Registration Number: NCT03147001
• Lead Sponsor: University of Aarhus

Brief Summary

A randomized interventions study using a crossover design. Ten well trained triathletes or bikeriders will be included for to undergo two trail separated by about 3 weeks. The night before the trail each subject will performe a high intense interval trainings session on an ergometer bike. during the night an infusion of aminoacid tracers will be initiated. The subject is woken at 6.30 a.m. Blood samples and a musclebiopsy will be collected prior to the morning bike ride at 8.00a.m. Immediately before the morning bike ride is started the subject will ingest a drink of 0,5g protein or a non-caloric placebo (random order). During the bike ride and the following 4,5 hours blood will be drawn with regular intervals. at time point 0, 60 and 180 min after the training session biopsies are collected.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-04-30
• Primary Completion: 2017-01-10
• Study Completion: 2017-01-10
• Status Verified: 2017-05
• Study First Submitted: 2017-05-08
• Study First Submitted QC: 2017-05-08
• Last Update Submitted: 2017-05-08
• Study First Posted: 2017-05-10
• Last Update Posted: 2017-05-10

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 9

Inclusion Criteria

• Healthy well-trained triathletes or cyclists
• VO2max > 55 ml O2/kg/min
• Training and has been training more than 7 times a week the last 6 months.

Read More

Exclusion Criteria

• VO2max < 55 ml O2/kg/min
• Has chronic disease
• has incjected with tracer on a previous occasion.

Read More

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Arm && Interventions

Group	Intervention	Description
Protein ingestion	Protein ingestion	Subjects ingested protein drinks
Placebo	Non-caloric placebo ingestion	Subjects ingested a non-caloric placebo drink

Primary Outcome Measures

Name	Time	Method
Muscle protein fractional synthetic rate (FSR)	FSR was measure over at time period of six hours

Secondary Outcome Measures

Name	Time	Method
Muscle signalling	Determined in biopsies sampled before and immediatly after 90min exercise in addition to one and four hours after exercise.	Muscle signalling will be determined using western blotting in each muscle biopsy
Nitrogen excretion	Urine is collected during two periods. Collection period one: 12 hours prior to morning exercise. Collection period 2: During and four hours following exercise. Blood samples is drawn repeatedly from before to four hours after exercise.	nitrogen excretion is determined by Plasma and urinary carbide analysis as well as plasma NH4+.
Tracer kinetics dilution model	six hours	A-V tracer dilution model was applied across the forearm.