A cluster investigation method for studying dengue virus genetic diversity, immunological responses and entomological dynamics

Completed

• Conditions: Dengue fever; Infections and Infestations; Dengue fever [classical dengue]

• Registration Number: ISRCTN21091116
• Lead Sponsor: niversity of Oxford (UK)

Brief Summary

Not available

Detailed Description

Not available

Study Timeline

• Study Start: 2008-07-17
• Last Update Posted: 13 January 2015

Recruitment & Eligibility

• Status: Completed
• Sex: All
• Target Recruitment: 200

Inclusion Criteria

Index cases:
1. All ages, either sex
2. Clinical suspicion of dengue:
2.1. Confirmed fever (i.e. an axillary temperature greater than 38°C at presentation)
2.2. Fever less than 72 hours duration
3. Positive NS1 antigen rapid test (indicative of viraemia)

Sample collection around index case:
All family members and neighbours

Exclusion Criteria

Does not meet inclusion criteria

Study & Design

• Study Type: Observational
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
To study dengue pathogenesis by comparing the genetic diversity of virus populations in dengue cases versus infecting mosquitoes and in asymptomatic subjects versus symptomatic cases of different severity

Secondary Outcome Measures

Name	Time	Method
To study dengue pathogenesis by:<br>1. Studying the evolution of dengue virus quasispecies during the course of illness<br>2. Studying antibody responses, viral load and antigenemia in asymptomatic and symptomatic dengue virus infections of varying severity