Assessment of Serum Leptin in Vitiligo Patients
Trial Snapshot
- Phase
- Not Applicable
- Status
- Not yet recruiting
- Sponsor
- Sohag University
- Enrollment
- 400
- Locations
- 1
- Primary Endpoint
- level of serum leptin
Study Overview
Brief Summary
Evaluation of serum leptin in vitiligo patients and control Assessing its correlation to the body mass index and disease dermographic data .
Detailed Description
, leptin is supposed to contribute to vitiligo pathogenesis through promoting the release of pro-inflammatory mediators such as tumor necrosis factor α "TNF," IL-2 and IL-6, as well as enhancing cytotoxic T cells "CD8" recruitment and inhibiting the functions of regulatory T cells "Tregs". Leptin can also aid in induction of oxidative stress by activating nicotinamide adenine dinucleotide phosphate (NADPH) oxidase• Venous blood samples will be taken from patients and controls to evaluate serum leptin: Morning samples will be taken after 12-h overnight fast in plain vacutainer tubes and directly centrifuged (1098 g for 15 min).
- Sera will be then collected in plain, capped, 5 ml centrifuge tubes "Eppendorf" and freezed at -20ᵒC in a well-monitored freezer until the analysis is performed• Serum leptin will be quantitatively assessed in vitro using DRG leptin Sandwich Enzyme Linked Immuno-Sorbent Assay "ELISA".
- ELISA will be performed according to manufacturer's guidelines where each run included a standard curve.
- Using the mean absorbance value for each sample, the corresponding concentration from the standard curve will be determined.
Study Design
- Study Type
- Interventional
- Allocation
- Randomized
- Intervention Model
- Parallel
- Primary Purpose
- Treatment
- Masking
- None
Eligibility Criteria
- Ages
- 18 Years to — (Adult, Older Adult)
- Sex
- All
- Accepts Healthy Volunteers
- No
Inclusion Criteria
- •All patients with vitiligo who are above the age of 18 years
Exclusion Criteria
- •Patients with other autoimmune diseases
Arms & Interventions
group A
obese with vitligo
Intervention: serum leptin (Diagnostic Test)
group b
obese without vitligo
Intervention: serum leptin (Diagnostic Test)
group C
non obese with vitiligo
Intervention: serum leptin (Diagnostic Test)
group D
non obese without vitiligo
Intervention: serum leptin (Diagnostic Test)
Outcomes
Primary Outcomes
level of serum leptin
Time Frame: 6 month
Evaluation of serum leptin in vitiligo patients and control Assessing its correlation to the body mass index and disease dermographic data .
Secondary Outcomes
No secondary outcomes reported
Investigators
Doaa Zaghloul Amin
dermatology and andrology resident in luxor international hospital
Sohag University