Anti-inflammatory Cells in Peritoneal Cavity

Not Applicable

Terminated

• Conditions: Chronic Renal Insufficiency; Ovarian Cysts
• Interventions: Other: peritoneal fluid and blood sample

• Registration Number: NCT03189316
• Lead Sponsor: University Hospital, Montpellier

Brief Summary

Some subsets of lymphocytes are able to inhibit immune response and thus, could be used to control auto-immune diseases and transplant reject. In mice, the main source of those anti-inflammatory lymphocytes is the peritoneal cavity. No data are available in human. This study aims at exploring the presence of those anti-inflammatory lymphocytes in human peritoneal cavity and at determine how to expand those cells.

Detailed Description

B cells will be collected during procedures planned for patients' standard care. Peritoneal fluid and peripheral blood of patients will be collected during coelioscopy for exploration of ovarian cysts or dialysis for treatment of chronic renal insufficiency.

Regulatory B cells (Breg cells) will be measured in peritoneal fluid and in peripheral blood after in vitro stimulation and assessment of intracellular interleukin-10 by flow cytometry. Proportion of Breg cells among B cells will be compared between peritoneal fluid and peripheral blood in both groups.

Different protocols to induce differentiation of B cells into Breg cells will be tested and compared.

Study Timeline

• Study First Submitted: 2017-05-30
• Study Start: 2017-06-06
• Study First Submitted QC: 2017-06-13
• Study First Posted: 2017-06-16
• Primary Completion: 2017-12-13
• Study Completion: 2017-12-13
• Status Verified: 2018-06
• Last Update Submitted: 2018-06-12
• Last Update Posted: 2018-06-13

Recruitment & Eligibility

• Status: TERMINATED
• Sex: All
• Target Recruitment: 10

Inclusion Criteria

Not provided

Exclusion Criteria

Not provided

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
subjects with ovarian cyst	peritoneal fluid and blood sample	Peritoneal fluid sample obtained from coelioscopy for exploration of ovarian cysts and blood sample
subjects with peritoneal dialysis	peritoneal fluid and blood sample	Peritoneal fluid sample obtained from peritoneal dialysis fluid of patients with chronic renal insufficiency and blood sample

Primary Outcome Measures

Name	Time	Method
relative percentage of regulatory B cells among B cells in peritoneal cavity compared to peripheral blood	up to 48 hour	Comparison of Breg cell in peritoneal cavity to Breg cell in peripheral blood B

Trial Locations

Locations (1)

CHU montpellier - department of rheumatology; 🇫🇷 Montpellier, France