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A Study to Assess the Safety, Tolerability and Efficacy of IONIS-AGT-LRx in Hypertensive Participants With Uncontrolled Blood Pressure

Phase 2
Completed
Conditions
Hypertension
Interventions
Drug: Placebo
Registration Number
NCT04714320
Lead Sponsor
Ionis Pharmaceuticals, Inc.
Brief Summary

The purpose of this study was to evaluate the effect of IONIS-AGT-LRx compared to placebo on seated automated office systolic blood pressure (SBP) from baseline to Study Day 85 in uncontrolled hypertensive participants on ≥ 3 antihypertensive medications and to evaluate the effect of IONIS-AGT-LRx on ambulatory blood pressure, seated automated office SBP, seated automated office diastolic blood pressure (DBP), and plasma angiotensinogen (AGT) at each scheduled visit in uncontrolled hypertensive participants on ≥ 3 antihypertensive medications.

Detailed Description

This was a phase 2, double-blind, randomized, placebo-controlled study in up to 160 participants. Participants were randomized in a 2:1 ratio and received a once-weekly subcutaneous (SC) treatment with either IONIS-AGT-LRx or matching placebo. The length of participation in the study was approximately 31 weeks, which included an up to 6-week screening period, a 12-week treatment period, and a 13-week post-treatment period.

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
160
Inclusion Criteria
  • Males or females aged 18-80 inclusive and weighing ≥ 50 kilograms (kg) at the time of informed consent
  • Females: must be non-pregnant and non-lactating, and either surgically sterile or post-menopausal
  • Males must be abstinent, surgically sterile or if engaged in sexual relations with a woman of childbearing potential (WOCBP), a highly effective contraceptive method must be used
  • Body mass index (BMI) ≤ 45.0 kilograms per square meter (kg/m^2)
  • At screening, the participant must have been on a stable, maximally tolerated regimen (per Investigator judgement) of 3 or more antihypertensive medications for at least 1 month prior to screening and will be required to maintain this regimen throughout the study. The combination of antihypertensive medications must be in the following categories: a) angiotensin-converting enzyme inhibitor (ACEi) or angiotensin II receptor blocker (ARB), b) beta blocker: c) calcium channel blocker d) diuretic, e) alpha-1 blocker f) centrally acting sympatholytic agent or g) direct acting vasodilators (e.g. hydralazine)
Exclusion Criteria
  • Clinically significant abnormalities in screening laboratory results, medical history according to Investigator judgment

  • History of secondary hypertension (HTN) including, but not limited to any of the following: renovascular HTN (unilateral or bilateral renal artery stenosis), coarctation of the aorta, primary hyperaldosteronism, Cushing's disease, pheochromocytoma, polycystic kidney disease, and drug-induced HTN

  • The use of the following at time of screening and during the course of the study:

    • Other medications for the treatment of HTN (e.g., minoxidil, diazoxide, renin inhibitors)
    • Medications that may cause hyperkalemia unless on a stable dose at least 1 month prior to the screening visit and no known history of hyperkalemia per Investigator judgement
    • Use of oral anticoagulants, unless stable for 4 weeks prior to the first dose of study drug and regular monitoring must be performed per clinical practice during the study unless the participant is receiving vitamin K agonists. If the participant is receiving vitamin K antagonists (e.g., warfarin) international normalized ratio (INR) should be in therapeutic range, as established by the Investigator, for 4 weeks prior to the first dose
    • Chronic administration of non-steroidal anti-inflammatory drugs (NSAIDs) or cyclooxygenase 2 (COX-2) inhibitors (except aspirin for cardiovascular disease provided the total daily dose does not exceed 325 mg)
  • History of bleeding diathesis, coagulopathy, immune thrombocytopenic purpura (ITP), thrombotic cytopenic purpura (TTP), or any qualitative or quantitative platelet defect

  • Unstable/underlying known cardiovascular disease defined as:

    • Any history of congestive heart failure (New York Heart Association [NYHA] Class III-IV)
    • Any history of previous myocardial infarction, coronary revascularization, unstable or stable angina pectoris ˂ 1 year prior to screening
    • Any hemodynamically unstable atrial or ventricular arrhythmias
    • Significant uncorrected valvular heart disease
    • Any history of stroke or transient ischemic attack < 1 year prior to screening
  • A cardiac valve repair, cardiac device implantation, and/or a hospitalization for heart failure within 3 months of screening

  • Participant works nighttime shifts (e.g., 11 PM to 7 AM)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
Pooled PlaceboPlaceboParticipants received ISIS 757456 matching placebo subcutaneously once weekly for 12 weeks.
IONIS-AGT-LRx 80 mgIONIS-AGT-LRxParticipants received ISIS 757456 80 milligrams (mg), subcutaneous (SC) injection, once weekly for 12 weeks.
IONIS-AGT-LRx 120 mgIONIS-AGT-LRxParticipants received ISIS 757456 120 mg, SC injection, once weekly for 12 weeks.
Primary Outcome Measures
NameTimeMethod
Change From Baseline in Seated Automated Office SBP to Day 85Baseline to Day 85
Secondary Outcome Measures
NameTimeMethod
Absolute Concentration of Plasma AGT at Each Scheduled, Post-Baseline VisitDays 15, 29, 43, 57, 71, 85, 92, 106, 120, 148, and 169
Change From Baseline in Plasma AGT to Each Scheduled, Post-Baseline VisitBaseline, Days 15, 29, 43, 57, 71, 85, 92, 106, 120, 148, and 169
Percent Change From Baseline in Plasma AGT at Each Scheduled, Post-Baseline VisitBaseline, Days 15, 29, 43, 57, 71, 85, 92, 106, 120, 148, and 169
Percentage of Participants Who Achieved Seated Automated SBP ≤ 130 mmHg, DBP ≤ 80 mmHg, and Both at Each Scheduled Post-Baseline VisitDays 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 120, 148, and 169
Change From Baseline to Study Day 85 in 24-hour Mean SBP and Diastolic Blood Pressure (DBP) Measured by Ambulatory Blood Pressure Monitoring (ABPM)Baseline, Day 85
Percentage of Participants Who Achieved Seated Automated Office SBP ≤ 140 mmHg, DBP ≤ 90 mmHg, and Both at Each Scheduled Post-Baseline VisitDays 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 120, 148, and 169
Change From Baseline in Seated Automated Office SBP to Each Scheduled, Post-Baseline VisitBaseline, Days 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 120, 148, and 169
Change From Baseline in Seated Automated Office DBP to Each Scheduled, Post-Baseline VisitBaseline, Days 8, 15, 22, 29, 36, 43, 50, 57, 64, 71, 78, 85, 92, 106, 120, 148, and 169

Trial Locations

Locations (50)

Nature Coast Clinical Research - Crystal River

🇺🇸

Crystal River, Florida, United States

Eagle Clinical Research

🇺🇸

Chicago, Illinois, United States

Holston Medical Group

🇺🇸

Kingsport, Tennessee, United States

Allied Biomedical Research Institute, Inc.

🇺🇸

Miami, Florida, United States

Palm Research Center, Inc.

🇺🇸

Las Vegas, Nevada, United States

Syed Research Consultants LLC

🇺🇸

Sheffield, Alabama, United States

Cardiology and Medicine Clinic

🇺🇸

Little Rock, Arkansas, United States

Cahaba Research, Inc.

🇺🇸

Pelham, Alabama, United States

Pinnacle Research Group

🇺🇸

Anniston, Alabama, United States

ALL Medical Research, LLC

🇺🇸

Cooper City, Florida, United States

Protenium Clinical Research, LLC

🇺🇸

Hurst, Texas, United States

Kalo Clinical Research

🇺🇸

Salt Lake City, Utah, United States

South Oklahoma Heart Research

🇺🇸

Oklahoma City, Oklahoma, United States

Central Research Associates, Inc.

🇺🇸

Birmingham, Alabama, United States

Creekside Endocrine Associates, PC

🇺🇸

Denver, Colorado, United States

Achieve Clinical Research, LLC

🇺🇸

Birmingham, Alabama, United States

National Research Institute - Huntington Park

🇺🇸

Huntington Park, California, United States

Clinical Trials Research

🇺🇸

Lincoln, California, United States

East Coast Institute for Research

🇺🇸

Jacksonville, Florida, United States

AMPM Research Clinic

🇺🇸

Miami Gardens, Florida, United States

Ocala Research Institute

🇺🇸

Ocala, Florida, United States

Manassas Clinical Research Center

🇺🇸

Manassas, Virginia, United States

Ecogene-21

🇨🇦

Chicoutimi, Quebec, Canada

York Clinical Research LLC

🇺🇸

Norfolk, Virginia, United States

Canvas Clinical Research

🇺🇸

Lake Worth, Florida, United States

Advanced Research Center

🇺🇸

Anaheim, California, United States

Advanced Research Institute Inc

🇺🇸

New Port Richey, Florida, United States

San Fernando Valley Health Institute

🇺🇸

Van Nuys, California, United States

RESPIRE Research

🇺🇸

La Mesa, California, United States

Chase Medical Research LLC

🇺🇸

Waterbury, Connecticut, United States

Summit Research Group, LLC

🇺🇸

Stow, Ohio, United States

Georgia Institute for Clinical Research

🇺🇸

Marietta, Georgia, United States

Gwinnett Research Institute

🇺🇸

Buford, Georgia, United States

Clinical Trials of America, LLC - Monroe, LA

🇺🇸

West Monroe, Louisiana, United States

Sandhill Research, LLC

🇺🇸

Decatur, Georgia, United States

NY Scientific

🇺🇸

Brooklyn, New York, United States

The Research Group of Lexington, LLC

🇺🇸

Lexington, Kentucky, United States

Clinical Investigation Specialists, Inc. - Wauconda

🇺🇸

Wauconda, Illinois, United States

Louisiana Heart Center

🇺🇸

Slidell, Louisiana, United States

Health Concepts Research

🇺🇸

Rapid City, South Dakota, United States

BioPharm Clinical Research

🇺🇸

Caro, Michigan, United States

Conrad Clinical Research

🇺🇸

Edmond, Oklahoma, United States

Juno Research, LL

🇺🇸

Houston, Texas, United States

TPMG Clinical Research

🇺🇸

Williamsburg, Virginia, United States

Chattanooga Research & Medicine, PLLC

🇺🇸

Chattanooga, Tennessee, United States

CardioVasc HR

🇨🇦

Saint-Jean-sur-Richelieu, Quebec, Canada

North Texas Research Associates

🇺🇸

Allen, Texas, United States

Laguna Clinical Research Associates

🇺🇸

Laredo, Texas, United States

Progressive Medical Research

🇺🇸

Port Orange, Florida, United States

Catalina Research Institute

🇺🇸

Montclair, California, United States

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