Study on Treating by Eating: Role of AGEs on Mucosal Barrier and Microbiome.
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 20
- 试验地点
- 1
- 主要终点
- Gut microbial composition
研究概览
简要总结
This is an exploratory study aimed at healthy volunteers to investigate the impact of differential cooking methods on global and gut inflammation, intestinal permeability, and the gut microbiota. The investigators hypothesize that a short dietary intervention of baking and grilling of food for 2 weeks as opposed to steaming and boiling of food for 2 weeks will result in measurable differences in blood, stool and urine samples that guide towards gut and overall health and disease. All food will be provided, together with detailed cooking instructions. Food logs including photographs will be used to check whether participants complied with the study.
The investigators aim to better understand the role of cooking methods in human health, so that this can be implemented in preventive strategies and dietary treatments for specific patient groups.
详细描述
This is a pilot project aimed at healthy volunteers to investigate the impact of differential cooking methods on systemic and intestinal inflammation, permeability, and the gut microbiota, using a randomised cross-over design. The investigators hypothesize that a short dietary intervention of baking and grilling of food for 2 weeks (high-Advanced Glycation End Products (AGEs) diet) as opposed to steaming and boiling of food for 2 weeks (low-AGEs diet) will result in measurable differences in inflammatory, cardiometabolic and microbial markers in blood and stool as well as changes in intestinal permeability through their effect on dietary AGEs. All food will be provided, together with detailed cooking instructions. Food logs including photographs will be used to assess and check adherence to the intervention.
The investigators aim to better understand the role of cooking methods and thermal treatments in human health, so that this can be implemented in preventive strategies and dietary treatments for specific patient groups.
研究设计
- 研究类型
- Interventional
- 分配方式
- Randomized
- 干预模型
- Crossover
- 主要目的
- Prevention
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 65 Years（Adult, Older Adult）
- 性别
- All
- 接受健康志愿者
- 是
入选标准
- •Overall healthy
- •Adhering to an omnivorous diet
- •Body Mass Index (BMI) between 18.5 - 30 kg/m2
- •Able to provide consent.
排除标准
- •Participants with chronic illnesses including diabetes mellitus, cardiovascular disease, or cancer; eating disorders, irritable bowel syndrome; inflammatory bowel diseases, and previous bowel surgery (except for appendectomy).
- •Current smoking
- •Participants taking nonsteroidal anti-inflammatory drugs (NSAIDS), proton pump inhibitors, antacids, or laxatives one month before enrollment or antibiotics, prebiotics or probiotics six months before the start of the trial.
- •Pregnancy of lactation
结局指标
主要结局
Gut microbial composition
时间窗: 2 weeks
Measurement of the composition of the gut microbiota using 16s rRNA sequencing of stool samples.
Intestinal transcellular permeability
时间窗: 2 weeks
Measurement of transcellular intestinal permeability using lipopolysaccharide binding protein in blood samples
Intestinal inflammation
时间窗: 2 weeks
Measurement of faecal calprotectin through stool samples (in mg/kg)
Systemic lipid profile
时间窗: 2 weeks
Total, LDL- and HDL-cholesterol values in blood samples (in mg/dl)
Weight changes
时间窗: 2 weeks
weight (in kg)
Gut microbial metabolism
时间窗: 2 weeks
Measurement of short chain fatty acids including acetic acid, propionic acid and butyric acid through stool samples (in mg/100g stool)
Intestinal paracellular permeability
时间窗: 2 weeks
Measurement of paracellular intestinal permeability using the lacultulose mannitol test using urine samples
Systemic cardiometabolic health
时间窗: 2 weeks
serum cardiometabolic parameters through OLINK cardiometabolic panel (measurement of 92 proteins in blood)
Handgrip strength changes
时间窗: 2 weeks
Handgrip strength (in kg)
Systemic inflammation
时间窗: 2 weeks
serum inflammation through OLINK inflammatory panel (measurement of 92 proteins in blood)
次要结局
未报告次要终点
研究者
JoaoSabino
Prof. dr. João Sabino
Universitaire Ziekenhuizen KU Leuven