The Role of Uterine NK Cells and T Cell Cytokines in Recurrent Miscarriage.
试验速览
- 阶段
- 不适用
- 状态
- 终止
- 发起方
- 入组人数
- 500
- 试验地点
- 1
- 主要终点
- the role of uterine natural killer cell(uNK)in recurrent pregnancy losses and the correlation between the numbers of it and embryo toxicity by measuring the level of the Th2 cytokines in normal reproductive profile and in patients with RPL.
研究概览
简要总结
The aim of the study is to evaluate the role of uterine natural killer cell(uNK)in recurrent pregnancy losses and the correlation between the numbers of it and embryo toxicity by measuring the level of the Th2 cytokines in normal reproductive profile and in patients with Recurrent Pregnancy Losses (RPL).
详细描述
Office hysteroscopy examination of the patients at the luteal phase of the natural cycle (day17 to day 20),with endometrial biopsy using pipple curette from the region of the fundus, the biopsy sent to flow cytometry laboratory in South Egypt Cancer Institute to be examined for:
1.1. Phenotypic analysis of uterine natural killer cells:
1.2. Induction and detection of intracellular cytokines by flow Cytometry:
研究设计
- 研究类型
- Interventional
- 分配方式
- Non Randomized
- 干预模型
- Single Group
- 主要目的
- Screening
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 40 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 是
入选标准
- •Women with unexplained recurrent miscarriages (2 or more consecutive).
- •All patients have normal uterine cavity diagnosed by HSG and /or hysteroscopy, normal semen analysis, normal thrombophilic profiles, no history of malformed
- •normal fetus in the previous miscarriages
排除标准
- •Age above forty years old .
- •Antiphospholipid syndrome (lupus anticoagulant and/or anticardiolipin antibodies [IgG or IgM]); other recognised thrombophilic conditions (testing according to usual clinic practice).
- •Intrauterine abnormalities (as assessed by ultrasound, hysterosonography, hysterosalpingogram, or hysteroscopy).
- •Fibroids distorting uterine cavity .
- •Abnormal parental karyotype .
- •Other identifiable causes of recurrent miscarriages (tests initiated only if clinically indicated) e.g., diabetes, thyroid disease and systemic lupus erythematosus (SLE).
研究组 & 干预措施
No treatment
patients with recurrent miscarriages
干预措施: office hysteroscopy (Device)
OFFICE HYSTEROSCOPY
Office hysteroscopic endometrial biopsy at the luteal phase of the menestrual cycle
干预措施: office hysteroscopy (Device)
结局指标
主要结局
the role of uterine natural killer cell(uNK)in recurrent pregnancy losses and the correlation between the numbers of it and embryo toxicity by measuring the level of the Th2 cytokines in normal reproductive profile and in patients with RPL.
时间窗: two years
次要结局
- to set in the horizon the role of immunomodulators as a treatment for RPL and implantation failure(two years)
研究者
alaa eldeen mahmoud ismail
Assistant professor ob/gyn,specialest of infertility and fetal medicine
Woman's Health University Hospital, Egypt