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A Study of 28431754 (Canagliflozin) on Gastrointestinal Glucose Absorption and Metabolism in Healthy Volunteers

Phase 1
Completed
Conditions
Healthy
Interventions
Other: no intervention
Drug: Canagliflozin/Placebo Placebo/Canagliflozin
Registration Number
NCT01173549
Lead Sponsor
Johnson & Johnson Pharmaceutical Research & Development, L.L.C.
Brief Summary

The purpose of this study is to evaluate the effect of canagliflozin on gastrointestinal glucose absorption in healthy volunteers.

Detailed Description

This is a single center study that will be conducted in two Parts (Part 1 and Part 2) to evaluate the effect of a single-dose of canagliflozin on the absorption of glucose in healthy volunteers. Part 1 is a pilot (small) study where responses to key pharmocodynamic (PD) parameters (ie, specific tests on blood samples to evaluate effects of no treatment on the body) will be measured to confirm the study design and number of volunteers planned for Part 2. Part 2 is the main study where volunteers will be randomized (assigned to study drug or placebo by chance) to receive double-blind (neither the volunteer or study physician will know the identity of the treatment assigned) treatment with canagliflozin or placebo (treatment identical in appearance to canagliflozin but contains no active drug) to evaluate the effect of canagliflozin compared to placebo on gastrointestinal glucose absorption using a radiolabeled glucose tracer approach (ie, where a radioactive substance is combined with glucose allowing the movement of glucose in the body to be traced or detected). Blood and urine samples will be collected from volunteers at pre-defined times during the study for PD analysis. Safety will be monitored during the study by evaluating adverse events and results from clinical laboratory tests, vital signs measurements, electrocardiograms (ECGs), and physical examinations performed. Volunteers in Part 1 will receive 240 ml of water 10 minutes before starting of the MMTT in each period (Periods 1 and 2). Volunteers in Part 2 will receive study drug 20 minutes prior to starting of the MMTT in treatment sequence 1 (a single 300-mg dose of canagliflozin in Period 1 followed by matching placebo in Period 2) or in treatment Sequence 2 (matching placebo in Period 1 followed by a single 300-mg dose of canagliflozin in Period 2). Periods 1 and 2 will be separated by 7 to 21 days.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
26
Inclusion Criteria
  • Have a Body Mass Index (BMI) between 20 and 27 kg/m2 (inclusive) and a body weight not less than 50 kg
  • Be healthy on the basis of physical examination including medical history, vital signs, 12-lead ECG, and all other screening lab tests performed at Screening and Admission
  • Agree (if men) to use a double barrier method of birth control (e.g., condom for them and use of spermacide with diaphragm, hormonal contraceptives or intrauterine devices by female partner) during the study and for three months following the last dose of study medication, and to not donate sperm during the study and for 3 months after receiving the last dose of study medication
  • Willing/able to adhere to the prohibitions and restrictions specified in this protocol
  • Must have signed an informed consent form.
Exclusion Criteria
  • Have a history of, or currently active, illness or medical condition or disorder that the Investigator considers to be clinically significant
  • Has been a smoker or tobacco user within the past 6 months
  • Have a history of recent major surgery (within 6 months of study start)
  • Tested positive for serology: hepatitis B surface antigen (HBsAg), hepatitis C antibodies (anti-HCV) or human immunodeficiency virus (HIV) antibodies
  • Fasting plasma glucose > 110 mg/dL
  • Have a history of alcohol or drug abuse within 2 years prior to Screening or a positive test for alcohol and/or drugs of abuse (including but not limited to barbiturates, opiates, cocaine, cannabinoids, amphetamines,MDMA (Ecstasy) and benzodiazepines) at Screening

Study & Design

Study Type
INTERVENTIONAL
Study Design
CROSSOVER
Arm && Interventions
GroupInterventionDescription
001no interventionno intervention Part 1: 240 mL water 10 minutes (min) prior to the start of the MMTT on Day 1 of Periods 1 and 2. Periods 1 and 2 will be separated by 7 to 21 days.
002Canagliflozin/Placebo Placebo/CanagliflozinCanagliflozin/Placebo Placebo/Canagliflozin Part 2: 240 mL water 20 min prior to the MMTT on Day 1 of Periods 1 and 2 in each treatment sequence (1 dose of canagliflozin in Period 1 followed by 1 dose of placebo in Period 2 and then crossover to 1 dose of placebo in Period 1 followed by 1 dose of canagliflozin in Period 2).
Primary Outcome Measures
NameTimeMethod
The amount of ingested glucose that is absorbed in systemic circulationDuring the first 1 and 2 hours after a standard mixed meal
Secondary Outcome Measures
NameTimeMethod
The amount of ingested glucose that is absorbed in systemic circulation2-6 hr and 0-6 hr after a standard mixed meal
Endogenous glucose production (EGP)At protocol-specified time points after a standard mixed meal
Total and incremental plasma glucose and insulin area under the curve (AUC)0-1, 0-2, 2-6, and 0-6 hours after a standard mixed meal
Gastric emptying: peak level, time to peak level, and AUCs0-6 hours after a standard mixed meal
Rate of Peripheral glucose disposal (Rd)At protocol-specified time points after a standard mixed meal
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