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Polscope Sperm: A Non-invasive Method to Assess DNA Damage in Individual Sperm

Completed
Conditions
DNA Damage
Other Complications Associated With Artificial Fertilization
Registration Number
NCT01461395
Lead Sponsor
University of South Florida
Brief Summary

The purpose of this study is to find a non-invasive way to discover DNA (deoxyribonucleic acid, which is the genetic material inside your cells) alterations in the sperm's head, in the near future leading us to choose the "good sperm" in a simplified manner. This could then increase chances of a successful in-vitro fertilization (IVF) treatment when there are sperm abnormalities.

Detailed Description

If you take part in this study,

1. Your physician is asking you to provide a semen sample as you already would. The sample will be taken to the IVF lab which would occur if you were not involved in this research. The part that would be thrown away is then further analyzed under a polarized light microscope. The sample is exposed to ultraviolet (UV) light for a period of 20 minutes after which it is analyzed under the microscope again, looking for any changes when compared the sample that was not exposed to UV light. The sample is then discarded.

2. Your name will be removed from the sample after the regular semen analysis is done; therefore there will be no way of associating the research results with you. No information will be needed from you and you will not be contacted in the future regarding this research.

Recruitment & Eligibility

Status
COMPLETED
Sex
Male
Target Recruitment
84
Inclusion Criteria
  • Age 18-60 years
  • provide informed consent
  • Already providing a semen sample as routine therapy in IVF
Exclusion Criteria

-unable to provide informed consent

Study & Design

Study Type
OBSERVATIONAL
Study Design
Not specified
Primary Outcome Measures
NameTimeMethod
Secondary Outcome Measures
NameTimeMethod

Trial Locations

Locations (1)

University of South Florida South Tampa Center

🇺🇸

Tampa, Florida, United States

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