The Effect of Acute Beetroot Juice Supplementation on Anaerobic Performance in Football Players

Not Applicable

Completed

• Conditions: Exercise Performance; Supplementation

• Registration Number: NCT07048912
• Lead Sponsor: Melike Nur Eroğlu

Brief Summary

The aim of this clinical trial is to examine the effects of two different natural fruit-vegetable blend beverages on short-duration, high-intensity exercise performance and muscle oxygenation in young male football players. The study also investigates how these beverages influence certain metabolic responses. The main questions it seeks to answer are:

* Does one of the beverages improve peak and mean power output during the Wingate test?

* How do these beverages affect muscle oxygenation and post-exercise recovery?

* Researchers will compare the effects of the two beverages on performance and metabolic markers.

Participants:

* Consume one of the two natural beverages 2.5 hours before testing (assigned at random)

* Perform a 30-second high-intensity Wingate cycling test

* Undergo measurements of muscle oxygenation, heart rate, blood pressure, blood lactate, and blood glucose during and after exercise

* Maintain consistent dietary and physical activity routines and avoid certain foods prior to testing

Detailed Description

Not available

Study Timeline

• Study Start: 2024-07-01
• Primary Completion: 2024-07-21
• Study Completion: 2024-07-25
• Status Verified: 2025-06
• Study First Submitted: 2025-06-11
• Study First Submitted QC: 2025-06-24
• Last Update Submitted: 2025-06-24
• Study First Posted: 2025-07-03
• Last Update Posted: 2025-07-03

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 16

Inclusion Criteria

• Healthy males over 18 years old
• Performing resistance exercise at least three times per week
• At least 5 years of football training experience

Exclusion Criteria

• Contraindications to Wingate exercise
• Presence of cardiometabolic disease
• Current use of ergogenic supplementation
• Smokers

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: CROSSOVER

Primary Outcome Measures

Name	Time	Method
Mean power output during the 30-second Wingate anaerobic test	Day 1 (2.5 hours after supplementation, during Wingate test)	Average power (in watts) generated during the 30-second Wingate cycling test will be compared between beetroot juice and placebo conditions to assess anaerobic performance.

Secondary Outcome Measures

Name	Time	Method
Muscle oxygen saturation (SmO₂)	Day 1 (pre-, during, and post-exercise on the day of Wingate test)	SmO₂ will be assessed using Near-Infrared Spectroscopy (NIRS) on the vastus lateralis muscle. Measurements will be taken continuously before, during, and after the 30-second Wingate anaerobic test to evaluate changes in muscle oxygenation in response to the intervention.

Trial Locations

Locations (1)

Sakarya University of Applied Sciences, Faculty of Sport Sciences, Exercise and Sports Sciences Education, Application and Research Centre; 🇹🇷 Sakarya, Turkey

Sakarya University of Applied Sciences, Faculty of Sport Sciences, Exercise and Sports Sciences Education, Application and Research Centre

🇹🇷Sakarya, Turkey