Testosterone-induced Muscle Memory and Underlying Mechanisms

Not Applicable

Not yet recruiting

• Conditions: Skeletal Muscle Physiology
• Interventions: Drug: Testosterone Undecanoate

• Registration Number: NCT06188767
• Lead Sponsor: Morten Hostrup, PhD

Brief Summary

The purpose of this project is to investigate underlying mechanisms of testosterone- and resistance training-induced muscle hypertrophy and muscle memory in humans.

Detailed Description

Not available

Study Timeline

• Status Verified: 2023-12
• Study First Submitted: 2023-12-18
• Study First Submitted QC: 2023-12-18
• Last Update Submitted: 2023-12-18
• Study First Posted: 2024-01-03
• Last Update Posted: 2024-01-03
• Study Start: 2024-09-01
• Primary Completion: 2025-09-01
• Study Completion: 2025-09-01

Recruitment & Eligibility

• Status: NOT_YET_RECRUITING
• Sex: Male
• Target Recruitment: 30

Inclusion Criteria

• Healthy men and women
• BMI of <26 and normal ECG and blood pressure

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Exclusion Criteria

• Smoking
• Chronic disease,
• Use of prescription medication
• Pain due to current or previous musculoskeletal injury
• Resistance training more than once per week in the 12 months leading up to the intervention
• Current or previous use of prohibited anabolic substances

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Study & Design

• Study Type: INTERVENTIONAL
• Study Design: PARALLEL

Arm && Interventions

Group	Intervention	Description
Placebo	Testosterone Undecanoate	Participants are randomized to a single placebo injection
Testosterone	Testosterone Undecanoate	Participants are randomized to a single injection of 1000 mg testosterone undecanoate

Primary Outcome Measures

Name	Time	Method
Myonuclei	The change in myonuclei is assessed at baseline, 4, 20 and 24 weeks	Immunohistochemistry to determine number of myonuclei
Fiber cross-sectional area	The change in fiber cross-sectional is assessed at baseline, 4, 20 and 24 weeks	Immunohistochemistry to determine fiber cross-sectional area
Skeletal muscle proteome	The change in the muscle proteome is assessed at baseline, 4, 20 and 24 weeks	Pathway enrichment analysis of the muscle proteome using mass spectrometry-based proteomics

Secondary Outcome Measures

Name	Time	Method
Lean mass	The change in lean mass is assessed at baseline, 4, 20 and 24 weeks	Lean mass assessed by dual X-ray absorptiometry (DXA)
Satellite cells	The change in satellite cells is assessed at baseline, 4, 20 and 24 weeks	Immunohistochemistry to determine number of satellite cells
Muscle strength	The change in 1-repetition maximum is assessed at baseline, 4, 20 and 24 weeks	1-repetition maximum