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Comparative Safety, Tolerability, Pharmacokinetic Study of AVT02 (100MG/ML) and Humira (100MG/ML) in Healthy Volunteers

Phase 1
Completed
Conditions
Healthy Volunteers
Interventions
Registration Number
NCT03579823
Lead Sponsor
Alvotech Swiss AG
Brief Summary

Adalimumab is an immunosuppressive drug that belongs to the family of anti-TNF agents. It contains a monoclonal antibody produced by biotechnology. It is designed to bind to tumor necrosis factor (TNF), a substance that is involved in several auto-immune processes. By binding to TNF, adalimumab blocks its activity, reducing the severity of various chronic inflammatory diseases including Rheumatoid Arthritis, Plaque Psoriasis and others.

Often, the high cost of biologic products may preclude access to the treatment to a big portion of the population worldwide. A biosimilar product that provides comparable safety and efficacy at more affordable cost would fulfill a broader medical need.

Humira has been available on the market for several years. Recently, a higher concentration (100 mg/mL) formulation has been introduced in major markets. Alvotech is developing AVT02, that is a proposed biosimilar of adalimumab containing high concentration (100 mg/mL) of active ingredient.

The objective of this clinical trial is to assess the similarity of AVT02 (100 mg/mL) with Humira (100 mg/mL), in terms of tolerability, safety (including immunogenicity) and compare the pharmacokinetics in healthy volunteers.

Detailed Description

Not available

Recruitment & Eligibility

Status
COMPLETED
Sex
All
Target Recruitment
24
Inclusion Criteria
  • Male and female healthy adult subjects willing to sign an Informed Consent Form and able to undergo protocol related procedures.
  • Age: 18 to 55 years, inclusive.
  • Body Mass Index (BMI): 19.0 to 30.0 kg per m2.
  • Medical history without major pathology, at the discretion of Principal Investigator.
  • Resting supine systolic blood pressure of ≤150 mmHg and diastolic blood pressure of ≤90 mmHg. Other vital signs showing no clinically relevant deviations according to the Principal Investigator's judgment.
  • Computerised 12-lead ECG recording without signs of clinically relevant pathology or showing no clinically relevant deviations as judged by the Principal Investigator.
  • Subjects who do not smoke tobacco products or use nicotine replacement therapy or e-cigarettes.

Main

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Exclusion Criteria
  • Evidence of clinically relevant pathology.
  • Unable to follow protocol instructions in the opinion of the Principal Investigator.
  • History of relevant drug and or food allergies.
  • History of allergic reactions attributed to compounds of similar chemical or biologic composition to agents used in study.
  • Known history of previous exposure to adalimumab or other anti-TNF-alpha molecules.
  • Any past or concurrent medical conditions potentially increasing the subject's risks, or would have interfered with the study evaluation, procedures, or study completion. Examples of these include medical history with evidence of clinically relevant pathology (e.g., malignancies, demyelinating disorders, herpes zoster, or hepatic, gallbladder or pancreatic diseases).
  • Presence of chronic obstructive pulmonary disease. Asthma in the childhood is allowed.
  • Evidence of a recent, within 6 months, infection requiring hospitalisation or intravenous antibiotic use.
  • Subject has a positive test for Tuberculosis (TB) during screening or a known history of active or latent TB, except documented and complete adequate treatment of TB.
  • Having received live vaccines during the 4 weeks before screening or have the intention to receive vaccination during the study.
  • Positive for Hepatitis B surface antigen (HBsAg), hepatitis B core antibody (HbcAb), anti- Hepatitis C virus antibodies (HCV), or anti-human immunodeficiency virus (HIV) 1 on 2 antibodies at screening.
  • Impaired liver function
  • Any persons who are an employee of the Principal Investigator, clinical centre, clinical research organisation or Sponsor, a relative of an employee of the clinical centre, the Investigator, Clinical Research Organization (CRO) or the Sponsor.
  • Other inclusion/exclusion criteria may apply.
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Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
AVT02 100 MG/MLAVT02 100MG/MLSingle subcutaneous injection of 40 mg of AVT02 (100MG/ML)
Adalimumab 100 MG/ML [HUMIRA]Adalimumab 100 MG/ML [Humira]Single subcutaneous injection of 40 mg of Adalimumab (100MG/ML) \[HUMIRA\]
Primary Outcome Measures
NameTimeMethod
Change from baseline red blood cells at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure red blood cells count, (unit/mm3)

Change from baseline blood chloride at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure chloride (mmol/L)

Change from baseline blood bicarbonate at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure bicarbonate (mmol/L)

Change from baseline blood creatinine at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure creatinine (micromol/L)

Change from baseline blood bilirubin at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure Bilirubin (micromol/L)

Change from baseline international normalised ratio at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure international normalised ratio

Change from baseline blood pressure at 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosingPredose and 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosing

Measurement of blood pressure (systolic and diastolic in mm Hg)

Change from baseline blood gamma glutamyl transferase at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure gamma glutamyl transferase (UI/L)

Change from baseline body temperature at 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosingPredose and 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosing

Measurement of oral temperature (Celsius degree)

Change from baseline haemoglobin at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure haemoglobin (g/L)

Change from baseline blood aspartate aminotransferase at 2, 3, 5, 9, 64 days postPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure aspartate aminotransferase (UI/L)

Change from baseline blood alanine aminotransferase at 2, 3, 5, 9, 64 days postPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure alanine aminotransferase (UI/L)

Change from baseline blood potassium at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure potassium (mmol/L)

Change from baseline blood sodium at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure sodium (mmol/L)

Change from baseline heart rate at 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosingPredose and 1, 2, 3, 4, 5, 6, 7, 8, 9, 64 days post dosing

Measure of heart rate (beats per minute)

Change from baseline white blood cells at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure white blood cells count, (unit/mm3)

Change from baseline platelets at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure platelets count, (unit/mm3)

Change from baseline blood calcium at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure calcium (mmol/L)

Change from baseline blood phosphate at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure phosphate (mmol/L)

Change from baseline electrocardiogram at 1, 2, 5, 9, 64 days post dosingPredose and 1, 2, 5, 9, 64 days post dosing

Analysis of 12-lead electrocardiogram

Change from baseline prothrombin time at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure prothrombin time (sec)

Change from baseline urine glucose at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Urine sample collection to measure glucose

Change from baseline urine protein at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Urine sample collection to measure protein

Change from baseline partial prothrombin time at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure partial prothrombin time (sec)

Change from baseline activated partial thromboplastin time at 2, 3, 5, 9, 64 days postPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure activated partial thromboplastin time (sec)

Change from baseline urine leucocytes at 2, 3, 5, 9, 64 days post dosingPredose and 2, 3, 5, 9, 64 days post dosing

Urine sample collection to measure leucocytes

Change from baseline thrombin time at 2, 3, 5, 9, 64 days postPredose and 2, 3, 5, 9, 64 days post dosing

Blood collection to measure thrombin time (sec)

Secondary Outcome Measures
NameTimeMethod
Area under the plasma concentration-time curve (AUC)Over 64 days

Venous blood samples will be collected for measurement of Area under the plasma concentration-time curve (AUC) of AVT02 and EU Humira

Maximum serum concentrationOver 64 days

Venous blood samples will be collected for measurement of serum concentration of AVT02 and EU Humira

Time to maximum serum concentrationOver 64 days

Evaluation of time to maximum plasma concentration (Tmax) of AVT02 and Humira.

Terminal half-lifeOver 64 days

Evaluation of terminal elimination half-life (T1/2) of AVT02 and Humira

Volume of distributionOver 64 days

Evaluation of volume of distribution during the elimination phase (Vz) of AVT02 and Humira

ClearanceOver 64 days

Evaluation of total plasma clearance (CL) of AVT02 and Humira

Incidence and titer of anti-drug antibodies to adalimumab15, 29 and 64 days after dosing

A blood sample will be collected to measure antibodies to AVT02 and Humira

Trial Locations

Locations (1)

Nucleus Network

🇦🇺

Melbourne, Victoria, Australia

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