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Trial to Evaluate Efficacy and Safety of Bortezomib in Patients with Severe Autoimmune Encephalitis

Phase 2
Recruiting
Conditions
Autoimmune Encephalitis
Interventions
Drug: Placebo
Registration Number
NCT03993262
Lead Sponsor
Jena University Hospital
Brief Summary

Autoimmune Encephalitis is a disorder of the central nervous system caused by bodily substances, called antibodies. Antibodies normally help the body to prevent infections. However, in this disorder, the antibodies turn against the body itself and especially against cells in the brain and disturb the normal brain function. They are therefore called autoantibodies.

There is no specific therapy for patients with autoimmune encephalitis so far. At the moment, the symptoms are treated with approved medications such as cortisone and immunotherapies also used in oncology. These therapies are unspecified and aim to reduce the number of autoantibodies and to contain the autoimmune process. In this trial we aim to test a new therapy option: in this therapy the body cells producing autoantibodies will be specifically targeted by a substance called bortezomib.

The trial addresses patients with severe autoimmune encephalitis. The aim of the trial is to evaluate the efficacy and safety of bortezomib in patients with severe autoimmune encephalitis.

Detailed Description

Autoimmune encephalitis is characterized by autoantibodies against neuronal surface antigens like the NMDA (N-methyl-D-aspartate) receptor or LGI1 (Leucin-rich glioma inactivated protein 1). So far, no specific therapy exists for this disease. Actual treatment includes combination therapies aiming for a reduction of pathogenic antibodies and containing the autoimmune process. In first line, patients are treated with plasmapheresis and cortisone. In second line, Rituximab and/or cyclophosphamide are administered. The response to these treatments are, however, often delayed and insufficient.

Therefore, we need a specific therapy aiming at the antibody-producing plasma cells.

Bortezomib is a proteasome inhibitor which interferes with NF-kB (nuclear factor kB) and the ubiquitin proteasome signaling pathway. Bortezomib acts preferably on cells with high protein synthesis - like plasma cells - and induces cell death in these cells. Bortezomib is used since more than a decade in chemotherapy of the multiple myeloma. Additionally, it is reported for systemic autoimmune diseases like lupus erythematodes that bortezomib leads to a depletion of plasma cells and therefore reduces the number of pathogenic antibodies and improves clinical outcome. The therapeutic potential of bortezomib for NMDAR encephalitis is described in a first case series with 5 patients.

Recruitment & Eligibility

Status
RECRUITING
Sex
All
Target Recruitment
50
Inclusion Criteria
  • Clinically diagnosed severe autoimmune encephalitis (defined as mRS ≥ 3) with autoantibodies to neuronal surface proteins in cerebrospinal fluid and / or serum
  • Pretreatment with rituximab
  • Age ≥18 years
  • signed informed consent
  • Women of childbearing potential (up to 2 years after menopause): negative pregnancy test
Exclusion Criteria
  • pregnancy/breast-feeding
  • acute infiltrative pulmonary and pericardial disease
  • malignant tumor under current chemotherapy
  • Simultaneous participation in another intervention study
  • Previous participation in this study
  • Known hypersensitivity to an ingredient of the investigational product
  • Continued therapy with glucocorticoids / rituximab during the study duration (last dose must be administered before the first dose of the investigational product)

Study & Design

Study Type
INTERVENTIONAL
Study Design
PARALLEL
Arm && Interventions
GroupInterventionDescription
PlaceboPlacebo1 to 3 cycles placebo (NaCl solution) s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)
InterventionalBortezomib1 to 3 cycles Bortezomib with 1,3mg/m2 body surface s.c. + 20mg dexamethasone p.o. on days 1, 4, 8 and 11 (= 1 cycle)
Primary Outcome Measures
NameTimeMethod
modified Rankin-Score (mRS)17 weeks after first administration of the study drug

modified Rankin-Score from 0 = no symptoms to 6 = death

Secondary Outcome Measures
NameTimeMethod
modified Rankin-Score (mRS)3, 6, 9 and 13 weeks after first administration of the study drug; GCS Score also 17 weeks after first administration of the study drug

modified Rankin-Score from 0 = no symptoms to 6 = death

Length of in-hospital stay / length of ICU stayuntil 17 weeks after first administration of the study drug

Number of days in hospital or on ICU for each patient from first administration of the study drug until 17 weeks after first administration of the study drug

Immune responseat study start and 17 weeks after first administration of the study drug

Antibody titer (in serum and liquor) and cellular immune response (FACS analysis of liquor)

neurocognitive function assessed by Montreal Cognitive Assessmentat study start and 17 weeks after first administration of the study medication

total score of the Montreal Cognitive Assessment (MoCA) (0 to max. 30 points = best possible result)

neurocognitive function assessed by Mini-Mental Status Testat study start and 17 weeks after first administration of the study medication

total score of the Mini-Mental Status Test (MMST) (0 to max 30 points = best possible result)

neurocognitive function assessed by Rey Auditory Verbal Learning Testat study start and 17 weeks after first administration of the study medication

total score of the Rey Auditory Verbal Learning Test (RAVLT) (memory performance assessed by 3 word lists which are read to the patient and should be recalled and repeated by the patient; different proceeding for the 3 word lists)

neurocognitive function assessed by Neuropsychiatric Inventory Questionnaireat study start and 17 weeks after first administration of the study medication

total score of the Neuropsychiatric Inventory Questionnaire (NPI) (0 = best score to max 36 (patient) or 60 (caregiver)

safety of Bortezomib regarding polyneuropathy, increase of liver enzymes and secondary infectionsuntil 17 weeks after first administration of the study drug

number of polyneuropathy cases, number of increased liver enzymes, number of secondary infections

safety of Bortezomib regarding polyneuropathyuntil 17 weeks after first administration of the study drug

number of polyneuropathy cases

safety of Bortezomib regarding increase of liver enzymesuntil 17 weeks after first administration of the study drug

number of increased liver enzyme values

Secondary infections due to Bortezomibuntil 17 weeks after first administration of the study drug

number of secondary infections

Hematotoxicity events due to Bortezomibuntil 17 weeks after first administration of the study drug

number of hematotoxicity events

Gastrointestinal toxicity due to Bortezomibuntil 17 weeks after first administration of the study drug

number of gastrointestinal toxicity events

total Glasgow Coma Scale (GCS)3, 6, 9, 13 and 17 weeks after first administration of the study drug

GCS from 3 to 15 points (sum of 3 subscores eye response (1 to 4 points), motor response (1 to 6 points), verbal response (1 to 5 points); highest score = best score; 1= worst score)

Destruction marker UCH-L1 (Ubiquitin carboxy-terminal hydrolase L1) in serum and liquorat baseline visit and 17 weeks after first administration of the study drug

Analysis of destruction marker UCH-L1 in serum and liquor

Destruction marker Neurofilament light chain (in serum and liquor)at baseline visit and 17 weeks after first administration of the study drug

Analysis of destruction marker Neurofilament light chain in serum and liquor

Destruction markers GFAP (glial fibrillary acidic protein) in serum and liquorat baseline visit and 17 weeks after first administration of the study drug

Analysis of destruction marker GFAP in serum and liquor

Destruction marker TAU proteins in serum and liquorat baseline visit and 17 weeks after first administration of the study drug

Analysis of destruction marker TAU in serum and liquor

Trial Locations

Locations (17)

Ludwig-Maximilians-Universität München, Klinikum Großhadern

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München, Bayern, Germany

Universitätsklinikum Würzburg

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Würzburg, Bayern, Germany

Medizinische Hochschule Hannover

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Hannover, Niedersachen, Germany

Charité - Universitätsmedizin Berlin, Klinik für Neurologie mit Experimenteller Neurologie

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Berlin, Germany

Ruhr-Universität Bochum, St. Josef Hospital, Klinik für Neurologie

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Bochum, Germany

University Hospital Düsseldorf, Clinic for Neurology

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Düsseldorf, Germany

Universitätsklinikum Erlangen, Neurologische Klinik

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Erlangen, Germany

Universitätsklinikum Essen (AöR), Klinik für Neurologie

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Essen, Germany

University Hospital Frankfurt (Main), Clinic for Neurology

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Frankfurt, Germany

Universitätsmedizin Greifswald, Klinik und Poliklinik für Neurologie

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Greifswald, Germany

Universitätsmedizin Göttingen Georg-August-Universität, Klinik für Neurologie

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Göttingen, Germany

Universitätsklinikum Jena, Sektion Translationale Neuroimmunologie, Klinik für Neurologie

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Jena, Germany

Klinik für Neurologie UKSH, Campus Kiel

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Kiel, Germany

Universitätsklinikum Leipzig, Klinik und Poliklinik für Neurologie

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Leipzig, Germany

Universitätsmedizin Mainz, Klinik und Poliklinik für Neurologie

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Mainz, Germany

Universitätsklinikum Münster Klinik für Neurologie

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Münster, Germany

Universitätsklinikum Ulm, Klinik für Neurologie Neurologische Ambulanz

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Ulm, Germany

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