Metabolic Fate Modifications of Saturated Fats After an Overfeeding

Not Applicable

Completed

• Conditions: Obesity; Lipid Metabolism; Inflammation

• Registration Number: NCT00905892
• Lead Sponsor: Centre de Recherche en Nutrition Humaine Rhone-Alpe

Brief Summary

The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31_palmitic acid, d31_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).

Detailed Description

Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31_palmitic acid, d31_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.

Study Timeline

• Study Start: 2005-09
• Study First Submitted: 2009-05-20
• Study First Submitted QC: 2009-05-20
• Study First Posted: 2009-05-21
• Primary Completion: 2009-12
• Study Completion: 2010-03
• Status Verified: 2011-06
• Last Update Submitted: 2011-06-22
• Last Update Posted: 2011-06-23

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 40

Inclusion Criteria

• 18 to 55 years old
• BMI 18 to 30 kg/m²
• Stable physical activity
• Safety subject during medical consultation

Exclusion Criteria

• Medical or surgical history which may affect energy expenditure (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
• Drug use that could affect energy expenditure (steroids, nicotine substitutes, thyroid hormones)
• Eating disorder
• Intensive sportive activity
• Subjects who Smoke
• Claustrophobic subjects

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Primary Outcome Measures

Name	Time	Method
Post prandial partitioning of exogenous lipids	Before and after overfeeding

Secondary Outcome Measures

Name	Time	Method
Genes expression in adipose and muscle tissues	Before and after overfeeding

Trial Locations

Locations (1)

Centre de recherche en nutrition humaine Rhone-Alpes; 🇫🇷 Pierre-Bénite, France