Metabolic Fate Modifications of Saturated Fats After an Overfeeding
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 发起方
- 入组人数
- 40
- 试验地点
- 2
- 主要终点
- Post prandial partitioning of exogenous lipids
研究概览
简要总结
The purpose of this study is to determine the partitioning of exogenous lipids in the postprandial period while a study of overfeeding. The method is based on the incorporation of a stable isotopic tracer (d31_palmitic acid, d31_C16) in lipoprotein triglycerides (TG-CHYLOMICRON and TG-VLDL) and in free fatty acids (FFA).
详细描述
Healthy overweight and lean young men are subjected to an overfeeding during 56 days which corresponds to a supplement of 761 Kcal/day. During two exploration days (before : D0 et after : D56 overfeeding) they have ingested a breakfast with tracer (d31_palmitic acid, d31_C16, 20mg/kg) and blood and urine samples were collected every hour of each exploration day. The enrichment in deuterium was measured by gas chromatography-organic mass spectrometry (GC-OMS) in palmitic acid pool of lipid fractions.
研究设计
- 研究类型
- Interventional
- 分配方式
- Na
- 干预模型
- Single Group
- 主要目的
- Basic Science
- 盲法
- None
入排标准
- 年龄范围
- 18 Years 至 55 Years（Adult）
- 性别
- Male
- 接受健康志愿者
- 是
入选标准
- •18 to 55 years old
- •BMI 18 to 30 kg/m²
- •Stable physical activity
- •Safety subject during medical consultation
排除标准
- •Medical or surgical history which may affect energy expenditure (renal -cardiovascular -hepatic- endocrine-inflammatory diseases)
- •Drug use that could affect energy expenditure (steroids, nicotine substitutes, thyroid hormones)
- •Eating disorder
- •Intensive sportive activity
- •Subjects who Smoke
- •Claustrophobic subjects
结局指标
主要结局
Post prandial partitioning of exogenous lipids
时间窗: Before and after overfeeding
次要结局
- Genes expression in adipose and muscle tissues(Before and after overfeeding)