Evaluation of Prolidase in Obstructive Sleep Apnea Syndrome

• Conditions: Obstructive Sleep-Apnea Syndrome

• Registration Number: NCT03563118
• Lead Sponsor: Sakarya University

Brief Summary

Obstructive sleep apnea (OSA) is a highly prevalent breathing disorder in sleep. We have aimed to evaluate the relationship between OSA and prolidase activity, the oxidative stress index (OSI), total antioxidative capacity (TAC), and total oxidative capacity (TOC) and the relationship between carotid intima media thickness (CIMT).

Detailed Description

This study was approved by the local ethics committee in accordance with the Helsinki Declaration. Written informed consent was received from the OSAS Subjects patients and control subjects before enrolment in the study. The patient and control cohorts were recruited from the Pulmonary Medicine Department, Medical Faculty, Yuzuncu Yıl University. Blood samples were analysed at the Biochemistry Laboratory of Harran University Medical Faculty TAC and TOS levels were measured by using an automated measurement method. TAC measurement method involves the production of a potent biological hydroxyl radical. Ferrous ion solution is mixed with hydrogen peroxide. Thus, it is possible to measure the anti-oxidative effect of the sample against the potent free radical reactions initiated by the production of the hydroxyl radical. TOS method is based on the oxidation of ferrous ion to ferric ion in the presence of various oxidant species in acidic medium and the measurement of the ferric ion by xylenol orange. mmol Trolox equivalent (equiv)/L, mmol H2O2 /L and mg/dL, respectively.

Study Timeline

• Status Verified: 2018-06
• Study First Submitted: 2018-06-07
• Study First Submitted QC: 2018-06-19
• Last Update Submitted: 2018-06-19
• Study First Posted: 2018-06-20
• Last Update Posted: 2018-06-20
• Study Start: 2018-07-01
• Primary Completion: 2018-09-01
• Study Completion: 2018-12-01

Recruitment & Eligibility

• Status: UNKNOWN
• Sex: All
• Target Recruitment: 74

Inclusion Criteria

• Patients were diagnosed with OSA after night polysomnography (PSG).
• Patients were enrolled in the study following receipt of their written informed consent.

Exclusion Criteria

• Patients had ischaemic cardiovascular diseases,
• Patients had chronic obstructive pulmonary diseases,
• Patients had ischaemic cerebral diseases,
• Patients had chronic inflammatory diseases,
• Patients had chronic and acute systemic infections.

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Primary Outcome Measures

Name	Time	Method
Prolidase activity	1 month	Prolidase activity was determined by a photometric method based on the measurement of the proline levels produced by prolidase (26). Plasma samples (100ml) were blended with 100 ml of serum physiological. A total of 25 ml of the mixture was preincubated with 75ml of the preincubation solution (50 mmol/l Tris HCl buffer pH 7.0 containing 1mmol/l glutathion, 50mmol/l MnCl2) at 37˚C for 30min.

Trial Locations

Locations (1)

Sakarya University Research and Training Hospital; 🇹🇷 Sakarya, Turkey