Androgen Receptor (AR) mRNA Expression is Positively Associated With Live Birth in Women Undergoing In-vitro Fertilization Independently of the Type of Ovarian Response
试验速览
- 阶段
- 不适用
- 状态
- 已完成
- 入组人数
- 67
- 主要终点
- Androgen Receptor mRNA expression
研究概览
简要总结
AR is a steroid hormone receptor that regulates various genes' expression and affects cellular proliferation and differentiation. Androgens interact with AR, exert their paracrine action on granulosa cells and regulate gonadotropin synthesis, follicle development, oocyte maturation and corpus luteum function. Estrogens increase AR mRNA and testosterone binding site in uterine endometrium and leiomyoma. In human cancer cells, low estradiol increases AR levels, suggesting the estrogen/androgen ratio as a predictor of sex steroid response. AR mRNA expression is, also, regulated by progesterone. Although AR mRNA is expressed in the female reproductive tissues, its expression has never been studied in peripheral blood.
研究设计
- 研究类型
- Observational
- 观察模型
- Case Control
- 时间视角
- Prospective
入排标准
- 年龄范围
- 20 Years 至 42 Years（Adult）
- 性别
- Female
- 接受健康志愿者
- 否
入选标准
- •Women with poor ovarian response according to bologna criteria
- •Advanced maternal age (≥40 years) or any other risk factor for poor ovarian response
- •A previous poor ovarian response (≤3 oocytes with a conventional stimulation protocol)
- •Abnormal ovarian reserve test, (AFC ˂5-7 follicles or AMH ˂0.5-1.1 ng/ml).
- •Women with normal ovarian response with normal menstrual cycles, normal astral follicle count and normal hormonal assay.
排除标准
- •Not more than 43 years old.
- •No history of malignancies, endometriosis or polycystic ovary syndrome.
- •Underlying genetic cause of infertility
- •History of severe cardiac, hepatic or renal disease.
- •History of systemic disease or treatment during the last three (3) months
- •Participation in another interventional study and a likelihood of being unavailable for follow-up.
结局指标
主要结局
Androgen Receptor mRNA expression
时间窗: day one, six and ten of ovarian stimulation
The expression of androgen receptor mRNA in peripheral blood of normal and poor responders level of expression in peripheral blood according to the Delta-Delta-Ct (ddCt) Algorithm. Percentage of increase?
次要结局
- Serum estradiol levels in peripheral blood of normal and poor responders(day one, six and ten of ovarian stimulation)
- Serum LH levels in peripheral blood of normal and poor responders(day one, six and ten of ovarian stimulation)
- Serum progesterone levels in peripheral blood of normal and poor responders.(day one, six and ten of ovarian stimulation)
研究者
E.M. Kolibianakis
Associate Professor
Aristotle University Of Thessaloniki