Analysis of microorganisms found in Root canal with Periapical lesions and evaluation of antimicrobial efficacy of combination of Calcium Hydroxide and Chitosan as Intracanal Medicaments.

Detailed case history including demographic details, medical history, oral health assessment status of the patients will be recorded. Radiographic examination will be done to evaluate the periapical status of the involved teeth. Based on the intracanal medicaments used, samples will be categorised in 3 groups:-

Group A - Calcium hydroxide alone

Group B - Calcium Hydroxide + Chlorhexidine

Group C - Calcium Hydroxide + Chitosan

Access opening will be done under rubber dam isolation after administration of local anesthesia. After locating the orifices, they are enlarged using orifice openers. First microbial samples will be collected using No 15/4% paper points keeping them within the canals for a minute. These paper points will be transferred to Institute’s Central Laboratory for microbiological assays and evaluation along with qualitative PCR. Intracanal medicaments will be placed inside the canals using lentulospirals and the patients are recalled after 14 days. In second appointment, after re-access opening, second microbial samples will be collected using No 15/4% paper points which will again be sent to the lab to identify the microorganisms along with its colony forming units to evaluate the decrease/increase in microbial load. Obturation will be done followed by composite access restoration. The obtained data will be subjected to statistical analysis. All the above procedures will be done after taking patients’ written consent.