Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging

Not Applicable

Completed

• Conditions: Flaccid Muscle Tone
• Interventions: Procedure: muscle biopsy

• Registration Number: NCT02685319
• Lead Sponsor: Purdue University

Brief Summary

Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging

Detailed Description

The aim of this study is to obtain human muscle samples for cell culturing to analyze muscle cell development in real-time to uncover factors that influence growth.

Study Timeline

• Study Start: 2016-02
• Study First Submitted: 2016-02-10
• Study First Submitted QC: 2016-02-12
• Study First Posted: 2016-02-18
• Primary Completion: 2016-05
• Study Completion: 2016-06
• Status Verified: 2017-06
• Last Update Submitted: 2017-06-19
• Last Update Posted: 2017-06-20

Recruitment & Eligibility

• Status: COMPLETED
• Sex: Male
• Target Recruitment: 7

Inclusion Criteria

• men 20-29 yrs
• BMI between 20 and 34.9
• Fasting Glucose <110 mg/dL
• Blood Pressure <160/100mm Hg
• Non-Diabetic
• LDL-Cholesterol <160mg/dL
• Triacylglycerol <400 mg/dL
• Makers of kidney, liver, and heart functions within 10% of clinical normalcy
• <1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

Exclusion Criteria

• men <20or>29 yrs

  • BMI between <20 and >34.9

  • Fasting Glucose >110 mg/dL

  • Blood Pressure >160/100mm Hg

  • Non-Diabetic

  • LDL-Cholesterol >160mg/dL

  • Triacylglycerol >400 mg/dL

  • Pre-existing kidney or liver conditions

    -->1.5hr /wk of habitual aerobic exercise training and no resistance exercise training in the past 6 months

  • Currently taking prescribed blood thinners

Study & Design

• Study Type: INTERVENTIONAL
• Study Design: SINGLE_GROUP

Arm && Interventions

Group	Intervention	Description
muscle biopsy	muscle biopsy	a muscle sample will be taken from the mid-thigh (Vastus Lateralis) and analyzed

Primary Outcome Measures

Name	Time	Method
Mapping Human Muscle Satellite Cell Fate By Real-Time Imaging	<10 hrs	this study is to obtain human muscle samples for cell culturing which will be exposed to a novel real-time imaging technique in order to elucidate factors that influence muscle satellite cell growth.

Trial Locations

Locations (1)

Purdue University - Stone Hall 700 W State St; 🇺🇸 West Lafayette, Indiana, United States