DNA Methylation-based Assays for Detecting Disease Spread in Rhabdomyosarcoma
Overview
- Phase
- Not Applicable
- Intervention
- Not specified
- Conditions
- Sarcoma
- Sponsor
- Children's Oncology Group
- Enrollment
- 170
- Primary Endpoint
- Panel of hypermethylation assays for minimal disseminated RMS
- Status
- Completed
- Last Updated
- 9 years ago
Overview
Brief Summary
RATIONALE: DNA analysis of tumor, blood, and bone marrow may help doctors predict if the disease has been disseminated.
PURPOSE: This research trial studies DNA tests in detecting disseminated disease in tumor, blood, and bone marrow samples from patients with rhabdomyosarcoma.
Detailed Description
OBJECTIVES: * To establish a panel of hypermethylation assays for minimal disseminated disease analysis in rhabdomyosarcoma (RMS). * To apply the hypermethylation assays to a series of bone marrow specimens from RMS patients. OUTLINE: Archived tumor, bone marrow, and peripheral blood samples are analyzed for DNA hypermethylation by PCR-based assays and microarray assays. Results are then compared with each patient's clinical and pathological characteristics, and outcomes. Patterns of locus hypermethylation in tumor and paired bone marrow samples are also compared.
Investigators
Eligibility Criteria
Inclusion Criteria
- Not provided
Exclusion Criteria
- Not provided
Outcomes
Primary Outcomes
Panel of hypermethylation assays for minimal disseminated RMS
Detection of RMS cells in bone marrow