Rheumatoid Arthritis Patients and Porphyromonas Gingivalis

Completed

• Conditions: Rheumatoid Arthritis
• Interventions: Procedure: blood sample; Procedure: synovial tissue

• Registration Number: NCT02905175
• Lead Sponsor: Centre Hospitalier Universitaire de Saint Etienne

Brief Summary

Rheumatoid arthritis (RA) is the most frequent joint inflammatory disease inducing joint destruction strongly correlated with anti-citrullinated protein antibodies citrullinated anti-peptide (ACPA). Precise RA etiology remained unclear with a described association between RA and periodontal disease. The missing link could be a gram-negative anaerobic bacterium called Porphyromonas gingivalis (P. gingivalis), which is heavily involved in periodontal disease and able to induce gingival citrullinated protein via an enzyme called peptidyl arginine deiminase (PAD). DNA of P. gingivalis was detected in synovial tissue and in peripheral blood mononuclear cell. To improve knowledge of P. gingivalis involvement in RA, the investigators will compare its effect on various cell type with comparison with another oral bacteria Provatella intermedia.

Detailed Description

Not available

Study Timeline

• Study Start: 2016-01-13
• Primary Completion: 2016-06-27
• Study Completion: 2016-06-27
• Study First Submitted: 2016-09-14
• Study First Submitted QC: 2016-09-16
• Study First Posted: 2016-09-19
• Status Verified: 2018-10
• Last Update Submitted: 2019-07-25
• Last Update Posted: 2019-07-26

Recruitment & Eligibility

• Status: COMPLETED
• Sex: All
• Target Recruitment: 11

Inclusion Criteria

• Age >18 ans
• RA or osteoarthritis with joint surgery planned
• Informed consent approved and signed

Exclusion Criteria

• Pregnancy

Study & Design

• Study Type: OBSERVATIONAL
• Study Design: Not specified

Arm && Interventions

Group	Intervention	Description
Rheumatoid arthritis	synovial tissue	blood sample specimen and synoviocytes from synovial tissue
Rheumatoid arthritis	blood sample	blood sample specimen and synoviocytes from synovial tissue
Osteoarthritis	blood sample	blood sample specimen and synoviocytes from synovial tissue
Osteoarthritis	synovial tissue	blood sample specimen and synoviocytes from synovial tissue

Primary Outcome Measures

Name	Time	Method
Comparison of genetic signature after induction by P. gingivalis and P. intermedia on synoviocytes	day 1

Secondary Outcome Measures

Name	Time	Method
Comparison of specific transcriptome profile induced by P. gingivalis compared to P. intermedia on peripheral blood mononuclear cells (PBMC) and gingival fibroblasts	day 1
Comparison of transcriptome profile between P. gingivalis and P. intermedia on RA synovial cells.	day 1
Comparison of the transcriptomic profile between RA and osteoarthritis synoviocytes	day 1

Trial Locations

Locations (1)

CHU Saint-Etienne; 🇫🇷 Saint-Etienne, France